Artículos de revistas
Proteomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous
Fecha
2011Registro en:
BMC Microbiology, Volumen 11,
14712180
14712180
10.1186/1471-2180-11-131
Autor
Martínez Moya, Pilar
Watt, Steven Alexander
Niehaus, Karsten
Alcaíno Gorman, Jennifer
Baeza Cancino, Marcelo
Cifuentes Guzmán, Víctor
Institución
Resumen
Background: The yeast Xanthophyllomyces dendrorhous is used for the microbiological production of the antioxidant carotenoid astaxanthin. In this study, we established an optimal protocol for protein extraction and performed the first proteomic analysis of the strain ATCC 24230. Protein profiles before and during the induction of carotenogenesis were determined by two-dimensional polyacrylamide gel electrophoresis and proteins were identified by mass spectrometry. Results: Among the approximately 600 observed protein spots, 131 non-redundant proteins were identified. Proteomic analyses allowed us to identify 50 differentially expressed proteins that fall into several classes with distinct expression patterns. These analyses demonstrated that enzymes related to acetyl-CoA synthesis were more abundant prior to carotenogenesis. Later, redox- and stress-related proteins were up-regulated during the induction of carotenogenesis. For the carotenoid biosynthetic enzymes mevalonate kinase and