Artículo de revista
Review: Placental syncytiotrophoblast membranes e domains, subdomains and microdomains
Fecha
2011Registro en:
Placenta 32, Supplement B, Trophoblast Research, Vol. 25 (2011) S196-S202
doi:10.1016/j.placenta.2011.01.002
Autor
Riquelme Pino, Gloria
Institución
Resumen
Human placental syncytiotrophoblast (STB) is an epithelium responsible for materno-fetal exchange. Ions
play multiple roles in STB, as in other transport epithelia. We have been interested in the character and
functional expression of ion channels in STB membrane fractions. Characterization of ion channels and
their relationship with different domains, subdomains and microdomains of STB membranes is important
to explain the intracellular mechanisms operating in the placental barrier. The aim of this paper is to
summarize our work on this subject. We isolated and purified basal membrane (BM) and two fractions
from the apical membrane, a classical fraction (MVM) and a light fraction (LMVM). They were used either
for reconstitution into giant liposomes or for transplantation into Xenopus oocytemembranes followed by
electrophysiological recordings to characterize chloride and cationic channels in STB from term human
placenta. In addition, Western blot analysis, using ion channel antibodies, was performed on purified
apical and basal membrane fractions.We also reported the presence of two functional microdomains (lipid
rafts) in LMVM and MVM, using detergent resistant membranes (DRMs) and cholesterol-sensitive
depletion.Moreover we found evidence of cytoskeletal participation in lipid rafts of different composition.
Our results contribute to knowledge of the ion channels present in STB membranes and their participation
in the physiology of this epithelium in normal and pathological pregnancies.