Artículo de revista
Stoichiometric Model and Metabolic Flux Analysis for Leptospirillum ferrooxidans
Fecha
2010-11-01Registro en:
Biotechnology and Bioengineering, Vol. 107, No. 4, November 1, 2010
0006-3592
DOI: 10.1002/bit.22851
Autor
Merino, M. P.
Andrews Farrow, Bárbara
Asenjo de Leuze, Juan
Institución
Resumen
A metabolic model for Leptospirillum ferrooxidans
was developed based on the genomic information of an
analogous iron oxidizing bacteria and on the pathways of
ferrous iron oxidation, nitrogen and CO2 assimilation based
on experimental evidence for L. ferrooxidans found in the
literature. From this metabolic reconstruction, a stoichiometric
model was built, which includes 86 reactions describing
the main catabolic and anabolic aspects of its
metabolism. The model obtained has 2 degrees of freedom,
so two external fluxes were estimated to achieve a determined
and observable system. By using the external oxygen
consumption rate and the generation flux biomass as input
data, a metabolic flux map with a distribution of internal
fluxes was obtained. The results obtained were verified with
experimental data from the literature, achieving a very good
prediction of the metabolic behavior of this bacterium at
steady state.