Artículo de revista
Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
Fecha
2008-12Registro en:
ELECTRONIC JOURNAL OF BIOTECHNOLOGY, Volume: 11,Issue: 5, Special Issue: Sp. Iss. SI Article Number: 1, 2008
0717-3458
Autor
Méndez, Marco
Rojas, Gabriela
Muñoz, Carlos
Lemus, Gamalier
Hinrichsen, Patricio
Institución
Resumen
The genetic characterization of 117 peach and nectarine
cultivars (Prunus persica (L.) Batsch) using
microsatellite (SSR) markers is presented. Analyzed
genotypes include the complete list of cultivars under
intellectual property (IP) protection in Chile. One
hundred and two out of the 117 cultivars under study
could be identified using only 7 SSRs. Other 5 cultivars
were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average
expected heterozygosity for the set of markers was 0.55,
ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022,
with an F value of 0.37. A Neighbor-Joining
dendrogram showed that, with few exceptions, peaches
and nectarines clustered separately. These results are
the basis for the development of a fingerprinting
protocol for the unequivocal identification of most of the
peach and nectarine cultivars officially registered in
Chile.