dc.creator | Del Campo Sfeir, Andrea Estefanía | |
dc.creator | Parra Ortíz, María Valentina | |
dc.creator | Vásquez Trincado, César Alonso | |
dc.creator | Gutiérrez, Tomás | |
dc.creator | Morales, Pablo E. | |
dc.creator | López Crisosto, Camila | |
dc.creator | Bravo Sagua, Roberto | |
dc.creator | Navarro Márquez, Mario F. | |
dc.creator | Verdejo, Hugo E. | |
dc.creator | Contreras Ferrat, Ariel Eduardo | |
dc.creator | Troncoso Cotal, Rodrigo | |
dc.creator | Chiong Lay, Mario | |
dc.creator | Lavandero González, Sergio | |
dc.date.accessioned | 2014-12-11T19:40:15Z | |
dc.date.available | 2014-12-11T19:40:15Z | |
dc.date.created | 2014-12-11T19:40:15Z | |
dc.date.issued | 2014 | |
dc.identifier | Am J Physiol Endocrinol Metab 306: E1–E13, 2014 | |
dc.identifier | doi:10.1152/ajpendo.00146.2013 | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/121889 | |
dc.description.abstract | Insulin is a major
regulator of glucose metabolism, stimulating its mitochondrial oxidation
in skeletal muscle cells. Mitochondria are dynamic organelles that
can undergo structural remodeling in order to cope with these everchanging
metabolic demands. However, the process by which mitochondrial
morphology impacts insulin signaling in the skeletal
muscle cells remains uncertain. To address this question, we silenced
the mitochondrial fusion proteins Mfn2 and Opa1 and assessed
insulin-dependent responses in L6 rat skeletal muscle cells. We found
that mitochondrial fragmentation attenuates insulin-stimulated Akt
phosphorylation, glucose uptake and cell respiratory rate. Importantly,
we found that insulin induces a transient rise in mitochondrial Ca2+
uptake, which was attenuated by silencing Opa1 or Mfn2. Moreover,
treatment with Ruthenium red, an inhibitor of mitochondrial Ca2+
uptake, impairs Akt signaling without affecting mitochondrial dynamics.
All together, these results suggest that control of mitochondrial
Ca2 uptake by mitochondrial morphology is a key event for insulininduced
glucose uptake. | |
dc.language | en | |
dc.publisher | American Physiological Society | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.subject | mitochondrial fragmentation | |
dc.title | Mitochondrial fragmentation impairs insulin-dependent glucose uptake by modulating Akt activity through mitochondrial Ca2+ uptake | |
dc.type | Artículos de revistas | |