Artículo de revista
Anomalous Dependence of Pyrene Spectra and Lifetimes with Temperature in Large Unilamellar Vesicles from Dioctadecyldimethylammonium Chloride and Dipalmito ylphosp hatidylcholine
Fecha
1992-08Registro en:
Langmuir 1992 v..8, No. 2 Pages:. 348-350
Autor
Lissi Gervaso, Eduardo A.
Abuin, Elsa
Sáez, M.
Zanocco Loyola, Antonio
Disalvo, A.
Institución
Resumen
Lifetimes of pyrene incorporated into large unilamellar vesicles of dipalmitoylphosphatidylcholine and
dioctadecyldimethylammonium chloride increase when the temperature increases prior to the melting
temperature of the bilayers. These changes are paralleled by a decrease in the vibronic bands intensity
ratio IIIIIII of the fluorescence spectra, indicative of water expulsion and/or a deeper penetration of the
probe prior to the bilayer phase transition. In DPPC vesicles, both pyrene lifetimes and the I ~ I IrIaIti os
show at the phase transition temperature changes that are indicative of increased water penetration.