Artículos de revistas
Identification of Tmem10/Opalin as an Oligodendrocyte Enriched Gene Using Expression Profiling Combined with Genetic Cell Ablation
Fecha
2008-06-20Registro en:
GLIA, Volume: 56, Issue: 11, Pages: 1176-1186, 2008
Autor
Golan, Neev
Adamsky, Konstantin
Kartvelishvily, Elena
Brockschnieder, Damian
Möbius, Wiebke
Spiegel, Ivo
Roth Metcalfe, Alejandro Darío
Thomson, Christine
Rechavi, Gideon
Peles, Elior
Institución
Resumen
Oligodendrocytes form an insulating multilamellar structure
of compact myelin around axons, which allows efficient
and rapid propagation of action potentials. However, little
is known about the molecular mechanisms operating at the
onset of myelination and during maintenance of the myelin
sheath in the adult. Here we use a genetic cell ablation
approach combined with Affymetrix GeneChip microarrays
to identify a number of oligodendrocyte-enriched genes that
may play a key role in myelination. One of the ‘‘oligogenes’’
we cloned using this approach is Tmem10/Opalin, which
encodes for a novel transmembrane glycoprotein. In situ
hybridization and RT-PCR analysis revealed that Tmem10
is selectively expressed by oligodendrocytes and that its
expression is induced during their differentiation. Developmental
immunofluorescence analysis demonstrated that
Tmem10 starts to be expressed in the white matter tracks
of the cerebellum and the corpus callosum at the onset of
myelination after the appearance of other myelin genes
such as MBP. In contrast to the spinal cord and brain,
Tmem10 was not detected in myelinating Schwann cells,
indicating that it is a CNS-specific myelin protein. In
mature oligodendrocytes, Tmem10 was present at the cell
soma and processes, as well as along myelinated internodes,
where it was occasionally concentrated at the paranodes.
In myelinating spinal cord cultures, Tmem10 was
detected in MBP-positive cellular processes that were
aligned with underlying axons before myelination commenced.
These results suggest a possible role of Tmem10
in oligodendrocyte differentiation and CNS myelination.