info:eu-repo/semantics/article
Naturally Occurring Compounds Elicit HIV-1 Replication in Chronically Infected Promonocytic Cells
Fecha
2014-05Registro en:
Barquero, Andrea Alejandra; Davola, Maria Eugenia; Riva, Diego Ariel; Mersich, Susana Esther; Alche, Laura Edith; Naturally Occurring Compounds Elicit HIV-1 Replication in Chronically Infected Promonocytic Cells; Hindawi Publishing Corporation; BioMed Research International; 2014; 5-2014; 1-6; 989101
2314-6133
CONICET Digital
CONICET
Autor
Barquero, Andrea Alejandra
Davola, Maria Eugenia
Riva, Diego Ariel
Mersich, Susana Esther
Alche, Laura Edith
Resumen
Since antiretroviral therapy suppresses but does not eradicate HIV-1 infection, methods to purge viral reservoirs are required. Many strategies involve the reactivation of chronically HIV infected cells to induce the expression of integrated viral genome. In this study, five bioactive compounds, the plant derivatives 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), nordihydroguaiaretic acid (NDGA), and curcumin (Cur) and the synthetic stigmasterol analogs (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (compound 2), were evaluated for their ability to elicit HIV replication in promonocytic (U1) and lymphocytic (H9+) HIV-1 chronically infected cells. The results revealed that natural compounds CDM, NDGA, and Cur were able to increase HIV-1 p24 antigen, determined by ELISA, only in latently infected promonocytic cells. CDM would reactivate HIV from latency by modulating the release of IL-6 and TNF-α, since the amount of both cytokines measured through ELISA significantly increased in U1 treated cells. Besides, NDGA increased ROS production, which might be related to the increase on p24 level observed in NDGA treated U1. These findings suggest that CDM, NDGA, and Cur might be candidates for further studies on latency-reversing therapeutics to eliminate latently HIV-1 reservoirs.