dc.creator | de la Torre, Eulalia | |
dc.creator | Hovsepian, Eugenia | |
dc.creator | Penas, Federico Nicolás | |
dc.creator | Dmytrenko, Ganna | |
dc.creator | Castro, Maria Ester | |
dc.creator | Goren, Nora Beatriz | |
dc.creator | Sales, Maria Elena | |
dc.date.accessioned | 2016-03-09T17:20:58Z | |
dc.date.accessioned | 2018-11-06T12:26:34Z | |
dc.date.available | 2016-03-09T17:20:58Z | |
dc.date.available | 2018-11-06T12:26:34Z | |
dc.date.created | 2016-03-09T17:20:58Z | |
dc.date.issued | 2013-01 | |
dc.identifier | de la Torre, Eulalia; Hovsepian, Eugenia; Penas, Federico Nicolás; Dmytrenko, Ganna; Castro, Maria Ester; et al.; Macrophages derived from septic mice modulate nitric oxide synthase and angiogenic mediators in the heart; Wiley; Journal of Cellular Physiology; 228; 7; 1-2013; 1584-1593 | |
dc.identifier | 0021-9541 | |
dc.identifier | http://hdl.handle.net/11336/4696 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1866667 | |
dc.description.abstract | Macrophages (Mps) can exert the defense against invading pathogens. During sepsis, bacterial lipopolisaccharide (LPS) activates the production of inflammatory mediators by Mps. Nitric oxide synthase (NOS) derived-nitric oxide (NO) is one of them. Besides, Mps may produce pro-angiogenic molecules such as vascular endothelial growth factor-A (VEGF-A) and metalloproteinases (MMPs). The mechanisms involved in the cardiac neovascular response by Mps during sepsis are not completely known. We investigated the ability of LPS-treated Mps from septic mice to modulate the behavior of cardiac cells as producers of NO and angiogenic molecules. In vivo LPS treatment (0.1 mg/mouse) increased NO production more than 4 fold and induced de novo NOS2 expression in Mps. Immunoblotting assays also showed an induction in VEGF-A and MMP-9 expression in lysates obtained from LPS-treated Mps, and in MMP-9 activity detected in cell supernatants. LPS-activated Mps co-cultured with normal heart induced the expression of CD31 and VEGF-A in heart homogenates and increased MMP-9 activity in the supernatants. By immunohistochemistry, we detected new blood vessel formation in hearts cultured with LPS treated Mps. When LPS-stimulated Mps were co-cultured with isolated cardiomyocytes in a transwell assay, the expression of NOS2, VEGF-A and MMP-9 was induced in cardiac cells. In addition, MMP-9 activity was up-regulated in the supernatant of cardiomyocytes. The latter was due to NOS2 induction in Mps from in vivo LPS-treated mice. In conclusion LPS-treated Mps are inducers of inflammatory/angiogenic mediators in cardiac cells, which could be triggering neovascularization, as an attempt to improve cardiac performance in sepsis. | |
dc.language | eng | |
dc.publisher | Wiley | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.24320/abstract | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1002/jcp.24320 | |
dc.relation | info:eu-repo/semantics/altIdentifier/issn/0021-9541 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | Macrophages | |
dc.subject | Nitric oxide synthase | |
dc.subject | Heart | |
dc.subject | LPS | |
dc.title | Macrophages derived from septic mice modulate nitric oxide synthase and angiogenic mediators in the heart | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |