dc.creator | TEIXEIRA-SA, Daniele M. A. | |
dc.creator | REICHER, Fany | |
dc.creator | BRAGA, Renata C. | |
dc.creator | BELTRAMINI, Leila Maria | |
dc.creator | MOREIRA, Renato de Azevedo | |
dc.date.accessioned | 2012-10-20T04:20:35Z | |
dc.date.accessioned | 2018-07-04T15:43:33Z | |
dc.date.available | 2012-10-20T04:20:35Z | |
dc.date.available | 2018-07-04T15:43:33Z | |
dc.date.created | 2012-10-20T04:20:35Z | |
dc.date.issued | 2009 | |
dc.identifier | PHYTOCHEMISTRY, v.70, n.17-18, p.1965-1972, 2009 | |
dc.identifier | 0031-9422 | |
dc.identifier | http://producao.usp.br/handle/BDPI/29908 | |
dc.identifier | 10.1016/j.phytochem.2009.08.011 | |
dc.identifier | http://dx.doi.org/10.1016/j.phytochem.2009.08.011 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1626548 | |
dc.description.abstract | A lectin and a galactoxyloglucan were characterized from Mucuna sloanei seed cotyledons. The galactoxyloglucan, isolated by water extraction and ethanol precipitation, had Glc:Xyl:Gal proportions in a molar ratio of 1.8:1.7:1.0 and a molar mass (M(w)) of 1.6 x 10(6) g mol(-1). The lectin (sloanin), isolated from the same seed by affinity chromatography on cross-linked Adenanthera pavonina galactomannan, gave two protein bands by SDS-PAGE (36 and 34 kDa) and one peak by gel filtration (63.6 kDa). Its N-terminal sequence indicated similar to 69% identity with soybean agglutinin to leguminous lectins. Circular dichroism (CD) spectra established that sloanin predominantly contains beta-sheet structures. Sloanin has similar to 5.5% carbohydrate and displayed hemagglutinating activity against rabbit and enzyme treated human erythrocytes, inhibited only by D-Gal containing sugars. The interaction between sloanin and storage cell-wall galactoxyloglucan was tested by affinity chromatography and fluorescence spectroscopy. (C) 2009 Elsevier Ltd. All rights reserved. | |
dc.language | eng | |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | |
dc.relation | Phytochemistry | |
dc.rights | Copyright PERGAMON-ELSEVIER SCIENCE LTD | |
dc.rights | restrictedAccess | |
dc.subject | Mucuna sloanei | |
dc.subject | Fabaceae | |
dc.subject | Galactoxyloglucan | |
dc.subject | Lectin | |
dc.subject | Sloanin | |
dc.title | Isolation of a lectin and a galactoxyloglucan from Mucuna sloanei seeds | |
dc.type | Artículos de revistas | |