Poloxamer 407/188 Binary Thermosensitive Hydrogels As Delivery Systems For Infiltrative Local Anesthesia: Physico-chemical Characterization And Pharmacological Evaluation

dc.creatorAkkari
dc.creatorAlessandra C. S.; Boava Papini
dc.creatorJuliana Z.; Garcia
dc.creatorGabriella K.; Dias Franco
dc.creatorMargareth K. K.; Cavalcanti
dc.creatorLeide P.; Gasperini
dc.creatorAntonio; Alkschbirs
dc.creatorMelissa Inger; Yokaichyia
dc.creatorFabiano; de Paula
dc.creatorEneida; Tofoli
dc.creatorGiovana R.; de Araujo
dc.creatorDaniele R.
dc.date2016
dc.datenov
dc.date2017-11-13T13:15:57Z
dc.date2017-11-13T13:15:57Z
dc.date.accessioned2018-03-29T05:53:21Z
dc.date.available2018-03-29T05:53:21Z
dc.identifierMaterials Science And Engineering: C-materials For Biological Applications. Elsevier Science Bv, v. 68, p. 299 - 307, 2016.
dc.identifier0928-4931
dc.identifier1873-0191
dc.identifierWOS:000382600000036
dc.identifier10.1016/j.msec.2016.05.088
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0928493116305215?via%3Dihub
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327452
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364477
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIn this study, we reported the development and the physico-chemical characterization of poloxamer 407 (PL407) and poloxamer 188 (PL188) binary systems as hydrogels for delivering ropivacaine (RVC), as drug model, and investigate their use in infiltrative local anesthesia for applications on the treatment of post-operative pain. We studied drug-micelle interaction and micellization process by light scattering and differential scanning calorimetry (DSC), the sol-gel transition and hydrogel supramolecular structure by small-angle-X-ray scattering (SAXS) and morphological evaluation by Scanning Electron Microscopy (SEM). In addition, we have presented the investigation of drug release mechanisms, in vitro/in vivo toxic and analgesic effects. Micellar dimensions evaluation showed the formation of PL407-PL188 mixed micelles and the drug incorporation, as well as the DSC studies showed increased enthalpy values for micelles formation after addition of PL 188 and RVC, indicating changes on self-assembly and the mixed micelles formation evoked by drug incorporation. SAXS studies revealed that the phase organization in hexagonal structure was not affected by RVC insertion into the hydrogels, maintaining their supramolecular structure. SEM analysis showed similar patterns after RVC addition. The RVC release followed the Higuchi model, modulated by the PL final concentration and the insertion of PL 188 into the system. Furthermore, the association PL407-PL188 induced lower in vitro cytotoxic effects, increased the duration of analgesia, in a single-dose model study, without evoking in vivo inflammation signs after local injection. (C) 2016 Elsevier B.V. All rights reserved.
dc.description68
dc.description299
dc.description307
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2014/26200-9, 2014/14457-5]
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [487619/2012-9, 309612/2013-6]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherElsevier Science BV
dc.publisherAmsterdam
dc.relationMaterials Science and Engineering: C-Materials for Biological Applications
dc.rightsfechado
dc.sourceWOS
dc.subjectPoloxamer
dc.subjectMicelle
dc.subjectHydrogel
dc.subjectRopivacaine
dc.subjectLocal Anesthesia
dc.titlePoloxamer 407/188 Binary Thermosensitive Hydrogels As Delivery Systems For Infiltrative Local Anesthesia: Physico-chemical Characterization And Pharmacological Evaluation
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución