Comparison Between Qualitative And Real-time Polymerase Chain Reaction To Evaluate Minimal Residual Disease In Children With Acute Lymphoblastic Leukemia.

dc.creatorPaula, Francisco Danilo Ferreira
dc.creatorElói-Santos, Silvana Maria
dc.creatorXavier, Sandra Guerra
dc.creatorGanazza, Mônica Aparecida
dc.creatorJotta, Patricia Yoshioka
dc.creatorYunes, José Andrés
dc.creatorViana, Marcos Borato
dc.creatorAssumpção, Juliana Godoy
dc.date
dc.date2016-05-23T19:43:48Z
dc.date2016-05-23T19:43:48Z
dc.date.accessioned2018-03-29T01:30:55Z
dc.date.available2018-03-29T01:30:55Z
dc.identifierRevista Brasileira De Hematologia E Hemoterapia. v. 37, n. 6, p. 373-380
dc.identifier1516-8484
dc.identifier10.1016/j.bjhh.2015.08.003
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/26670399
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/236063
dc.identifier26670399
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1304306
dc.descriptionMinimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia. The aim of this study was to analyze minimal residual disease using immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements by conventional polymerase chain reaction followed by homo-heteroduplex analysis and to compare this with real-time polymerase chain reaction at the end of the induction period in children with acute lymphoblastic leukemia. Seventy-four patients diagnosed with acute lymphoblastic leukemia were enrolled. Minimal residual disease was evaluated by qualitative polymerase chain reaction in 57 and by both tests in 44. The Kaplan-Meier and multivariate Cox methods and the log-rank test were used for statistical analysis. Nine patients (15.8%) were positive for minimal residual disease by qualitative polymerase chain reaction and 11 (25%) by real-time polymerase chain reaction considering a cut-off point of 1×10(-3) for precursor B-cell acute lymphoblastic leukemia and 1×10(-2) for T-cell acute lymphoblastic leukemia. Using the qualitative method, the 3.5-year leukemia-free survival was significantly higher in children negative for minimal residual disease compared to those with positive results (84.1%±5.6% versus 41.7%±17.3%, respectively; p-value=0.004). There was no significant association between leukemia-free survival and minimal residual disease by real-time polymerase chain reaction. Minimal residual disease by qualitative polymerase chain reaction was the only variable significantly correlated to leukemia-free survival. Given the difficulties in the implementation of minimal residual disease monitoring by real-time polymerase chain reaction in most treatment centers in Brazil, the qualitative polymerase chain reaction strategy may be a cost-effective alternative.
dc.description37
dc.description373-380
dc.languageeng
dc.relationRevista Brasileira De Hematologia E Hemoterapia
dc.relationRev Bras Hematol Hemoter
dc.rightsfechado
dc.sourcePubMed
dc.subjectAcute Lymphoblastic Leukemia
dc.subjectLeukemia Free Survival
dc.subjectMinimal Residual Disease
dc.subjectPolymerase Chain Reaction
dc.titleComparison Between Qualitative And Real-time Polymerase Chain Reaction To Evaluate Minimal Residual Disease In Children With Acute Lymphoblastic Leukemia.
dc.typeArtículos de revistas


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