Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms

dc.creatorSeneviratne, CJ
dc.creatorSilva, WJ
dc.creatorJin, LJ
dc.creatorSamaranayake, YH
dc.creatorSamaranayake, LP
dc.date2009
dc.dateNOV
dc.date2014-11-16T11:51:49Z
dc.date2015-11-26T17:24:44Z
dc.date2014-11-16T11:51:49Z
dc.date2015-11-26T17:24:44Z
dc.date.accessioned2018-03-29T00:12:01Z
dc.date.available2018-03-29T00:12:01Z
dc.identifierArchives Of Oral Biology. Pergamon-elsevier Science Ltd, v. 54, n. 11, n. 1052, n. 1060, 2009.
dc.identifier0003-9969
dc.identifierWOS:000271439500011
dc.identifier10.1016/j.archoralbio.2009.08.002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54854
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54854
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54854
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284153
dc.descriptionThe human fungal pathogen Candida is able to form biofilms in almost all the medical devices in cur-rent use. indeed, biofilm formation is a major virulence attribute of microorganisms and account for a majority of human infections. Therefore, understanding processes appertaining to biofilm development is an important prerequisite for devising new strategies to prevent or eradicate biofilm-related infections. In the present study we used an array of both conventional and novel analytical tools to obtain a comprehensive view of Candida biofilm development. Enumeration of colony forming units, colorimetric (XTT) assay, Scanning Electron Microscopy (SEM) and novel Confocal Laser Scanning Microscopy (CLSM) coupled with COMSTAT software analyses were utilised to evaluate growth kinetics; architecture and viability of biofilms of a reference (ATCC) and a clinical strain each of two Candida species, C. albicans and C. glabrata. Biofilm growth kinetics on a polystyrene substrate was evaluated from the initial adhesion step (1.5 h) up to 72 h. These analyses revealed substantial inter- and intra-species differences in temporal Organisation of Candida biofilm architecture, spatiality and cellular viability, while reaching maturity within a period of 48 h, on a polystyrene substrate. There were substantial differences in the growth kinetics upon methodology, although general trend seemed to be the same. Detailed architectural analysis provided by COMSTAT software corroborated the SEM and CSLM views. These analyses may provide a strong foundation for down stream molecular work of fungal biofilms. (C) 2009 Elsevier Ltd. All rights reserved.
dc.description54
dc.description11
dc.description1052
dc.description1060
dc.descriptionHong Kong Research [HKU 7624/06M]
dc.descriptionHong Kong Research [HKU 7624/06M]
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationArchives Of Oral Biology
dc.relationArch. Oral Biol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCandida
dc.subjectBiofilm
dc.subjectArchitecture
dc.subjectViability
dc.subjectGrowth kinetics
dc.subjectMaterials In-vitro
dc.subjectAntifungal Susceptibility
dc.subjectDietary Sugars
dc.subjectSurface
dc.subjectCells
dc.subjectFilamentation
dc.subjectParapsilosis
dc.subjectResistance
dc.subjectSerum
dc.subjectAssay
dc.titleArchitectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms
dc.typeArtículos de revistas


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