The influence of enamel matrix derivative associated with insulin-like growth factor-I on periodontal ligament fibroblasts

dc.creatorPalioto, DB
dc.creatorColetta, RD
dc.creatorGraner, E
dc.creatorJoly, JC
dc.creatorde Lima, AFM
dc.date2004
dc.dateAPR
dc.date2014-11-16T03:50:41Z
dc.date2015-11-26T16:19:24Z
dc.date2014-11-16T03:50:41Z
dc.date2015-11-26T16:19:24Z
dc.date.accessioned2018-03-28T23:02:25Z
dc.date.available2018-03-28T23:02:25Z
dc.identifierJournal Of Periodontology. Amer Acad Periodontology, v. 75, n. 4, n. 498, n. 504, 2004.
dc.identifier0022-3492
dc.identifierWOS:000221157400001
dc.identifier10.1902/jop.2004.75.4.498
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73477
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/73477
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73477
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267697
dc.descriptionBackground: Enamel matrix derivative (EMD) has recently been shown to promote periodontal regeneration in vivo. Insulinlike growth factor-I (IGF-I) is a potent modulator of periodontal regeneration stimulating cell proliferation, differentiation, synthesis of type I collagen, and non-collagenous proteins. However, the biochemical effects of these factors on periodontal ligament (PDL) fibroblasts are not completely understood. The objective of the present study was to evaluate the effect of EMD, IGF-I, and the combination of these two factors on the proliferation, adhesion, migration, and expression of type I collagen in PDL fibroblasts. Methods: The proliferation rate was measured by automated cell counting and immunohistochemical expression of proliferating cell nuclear antigen (PCNA). The cell adhesion was analyzed by a colorimetric assay and cell migration was measured in Boyden chambers. Type I collagen expression and production was determined by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results: The results indicated that the proliferation of PDL fibroblasts was significantly stimulated by EMD and EMD plus IGF-I in a dose- and time-dependent manner. EMD, IGF-I, and the combination of both factors had no effects on cellular migration and adhesion or expression and production of type I collagen. Conclusion: Our results showed that EMD, IGF-I, and the combination of both factors stimulated PDL fibroblast proliferation, whereas these factors did not affect adhesion, migration, or expression of type I collagen of these cells.
dc.description75
dc.description4
dc.description498
dc.description504
dc.languageen
dc.publisherAmer Acad Periodontology
dc.publisherChicago
dc.publisherEUA
dc.relationJournal Of Periodontology
dc.relationJ. Periodont.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcell division
dc.subjectenamel matrix derivative
dc.subjectfibroblasts, periodontal
dc.subjectgrowth factors, insulin-like
dc.subjectperiodontal ligament
dc.subjectHereditary Gingival Fibromatosis
dc.subjectTransforming Growth-factor-beta-1
dc.subjectFactors Invitro
dc.subjectHuman Cementum
dc.subjectCell-adhesion
dc.subjectRegeneration
dc.subjectAttachment
dc.subjectProtein
dc.subjectProliferation
dc.subjectCombination
dc.titleThe influence of enamel matrix derivative associated with insulin-like growth factor-I on periodontal ligament fibroblasts
dc.typeArtículos de revistas


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