Immunocytochemical Localization Of Tubulins In Spermatids And Spermatozoa Of Euptoieta Hegesia (lepidoptera: Nymphalidae)

dc.creatorMancini K.
dc.creatorBao S.N.
dc.creatorFernandes A.P.
dc.creatorDolder H.
dc.date2005
dc.date2015-06-26T14:06:32Z
dc.date2015-11-26T15:40:39Z
dc.date2015-06-26T14:06:32Z
dc.date2015-11-26T15:40:39Z
dc.date.accessioned2018-03-28T22:49:07Z
dc.date.available2018-03-28T22:49:07Z
dc.identifier
dc.identifierTissue And Cell. , v. 37, n. 2, p. 81 - 89, 2005.
dc.identifier408166
dc.identifier10.1016/j.tice.2004.10.008
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-14544268958&partnerID=40&md5=9ae2d0844a02ac06fc8f7d2935c5bce3
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/93148
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/93148
dc.identifier2-s2.0-14544268958
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1264422
dc.descriptionA comparative analysis of the distribution of tubulin types in apyrene and eupyrene sperm of Euptoieta hegesia butterflies was carried out, also verifying the presence of tubulin in lacinate appendages of the eupyrene sperm. Ultrathin sections of LR White embedded spermatids and spermatozoa were labeled for alpha, beta, gamma, alpha-acetylated and alpha-tyrosinated tubulins. Apyrene and eupyrene spermatids show the same antibody recognition pattern for tubulins. All tubulin types were detected in axonemal microtubules. Alpha and gamma tubulins were also detected on the cytoplasmic microtubules. However, for beta and tyrosinated tubulins only scattered labeling was detected on cytoplasmic microtubules and acetylated tubulin was not detected. In apyrene and eupyrene spermatozoa only the axoneme labeling was analyzed since cytoplasmic microtubules no longer exist in these cells. Alpha, beta and tyrosinated tubulins were easily detected on the apyrene and eupyrene axoneme; gamma tubulin was strongly marked on eupyrene axonemes but was scattered on the apyrene ones. Acetylated tubulin appeared with scattered labeling on the axoneme of both sperm types. Our results demonstrate significant differences in tubulin distribution in apyrene and eupyrene axonemal and cytoplasmic microtubules. Extracellular structures, especially the lacinate appendages, were not labeled by antibodies for any tubulin. © 2004 Elsevier Ltd. All rights reserved.
dc.description37
dc.description2
dc.description81
dc.description89
dc.descriptionArregui, C.O., Barra, H.S., Segmented pattern of tyrosinated microtubules assembly in neuritis of chick retinal neurons (1995) Biocell, 19, pp. 49-55
dc.descriptionBulinski, J.C., Gundersen, G.G., Stabilization and post-translational modification of microtubules during cellular morphogenesis (1991) Bioessays, 13, pp. 285-293
dc.descriptionCook, P.A., Wedell, N., Ejaculate dynamics in butterflies: A strategy for maximizing fertilization success (1996) Proc. R. Soc. Lond. B, 263, pp. 1047-1051
dc.descriptionCook, P.A., Wedell, N., Non-fertile sperm delay female remating (1999) Nature, 397, p. 486
dc.descriptionDallai, R., Afzelius, B.A., Microtubular diversity in insect spermatozoa: Results obtained with a new fixative (1990) J. Struct. Biol., 103, pp. 164-179
dc.descriptionDelgado-Viscogliosi, P., Brugerolle, G., Viscogliosi, E., Tubulin post-translational modifications in the primitive protist Trichomonas vaginalis (1996) Cell Motil. Cytoskel., 33, pp. 288-297
dc.descriptionDrummond, B.A., Multiple mating and sperm competition in the lepidoptera (1984) Sperm Competition and the Evolution of Animal Mating Systems, pp. 291-370. , R.L. Smith (Ed.). Academic Press London
dc.descriptionFernandes, A.P., Báo, S.N., Ultrastructural study of the spermiogenesis and localization of tubulin in spermatid and spermatozoon of Diabrotica speciosa (Coleoptera: Chrysomelidae) (1996) Cytobios, 86, pp. 231-241
dc.descriptionFernandes, A.P., Báo, S.N., Immunoelectron microscopy detection of tubulins during the spermiogenesis of phytophagous bugs (Hemiptera: Pentatomidae) (2001) Invert. Reprod. Dev., 40, pp. 163-170
dc.descriptionFosket, D.E., Morejohn, L.C., Structural and functional organization of tubulin (1992) Annu. Rev. Plant Physiol. Plant Mol. Biol., 43, pp. 201-240
dc.descriptionFrança, F.G.R., Báo, S.N., Dimorphism in spermatozoa of Anticarsia gemmatalis Hübner, 1918 (Insecta, Lepidoptera, Noctuidae) (2000) Braz. J. Morphol. Sci., 17, pp. 5-10
dc.descriptionFriedländer, M., The role of transient perinuclear microtubules during spermiogenesis of the warehouse moth Ephestia cautella (1976) J. Submicrosc. Cytol., 8, pp. 319-326
dc.descriptionFriedländer, M., Gershon, J., Reaction of surface lamella of moth spermatozoa to vinblastine (1978) J. Cell Sci., 30, pp. 353-361
dc.descriptionFriedländer, M., Gitay, H., The fate of the normal enucleated spermatozoa in inseminated female of the silkworm Bombyx mori (1972) J. Morphol., 138, pp. 121-129
dc.descriptionFuller, S.D., Gowen, B.E., Reinsch, S., Sawyer, A., Buendia, B., Wepf, R., Karsenti, E., The core of the mammalian centriole contains gamma-tubulin (1995) Curr. Biol., 5, pp. 1384-1393
dc.descriptionGage, M.J.G., Associations between body size, mating pattern, testis size and sperm lengths across butterflies (1994) Proc. R. Soc. Lond. B, 258, pp. 247-254
dc.descriptionGarvey, L.K., Gutierrez, G.M., Krider, H.M., Ultrastructure and morphogenesis of the apyrene and eupyrene spermatozoa in the gypsy moth (2000) Ann. Entomol. Soc. Am., 93, pp. 1147-1155
dc.descriptionGundersen, G.G., Kalmoski, M.H., Bulinski, J.C., Distinct populations of microtubules: Tyrosinated and nontyrosinated alpha-tubulin are distributed differently in vivo (1984) Cell, 38, pp. 779-789
dc.descriptionHuitorel, P., Audebert, S., White, D., Cosson, J., Gagnon, C., Role of tubulin epitopes in the regulation of flagellar motility (1999) The Male Gamete: From Basic Knowledge to Clinical Application, pp. 475-494. , C. Gagnon (Ed.). Cache River Press
dc.descriptionHuitorel, P., White, D., Fouquet, J.-P., Kann, M.-L., Cosson, J., Gagnon, C., Differential distribution of glutamylated tubulin isoforms along the sea urchin sperm axoneme (2002) Mol. Reprod. Dev., 62, pp. 139-148
dc.descriptionJamieson, B.G.M., Dallai, R., Afzelius, B.A., (1999) Insects: Their Spermatozoa and Phylogeny, , Enfield: New Hampshire (USA) Science Publishers, Inc
dc.descriptionJohnson, K.A., The axonemal microtubules of the Chamydomonas flagellum differ in tubulin isoform content (1998) J. Cell Sci., 111, pp. 313-320
dc.descriptionJoshi, H.C., Microtubules organizing center and gamma-tubulin (1994) Curr. Opin. Cell Biol., 6, pp. 55-62
dc.descriptionKatsuno, S., Studies on eupyrene and apyrene spermatozoa in the silkworm Bombyx mori L. (Lepidoptera: Bombycidae) III. The post-testicular behavior of the spermatozoa at various stages from pupa to the adult (1977) Appl. Entomol. Zool., 12, pp. 241-247
dc.descriptionKierszenbaum, A., Sperm axoneme: A tale of tubulin posttranslation diversity (2002) Mol. Reprod. Dev., 62, pp. 1-3
dc.descriptionKubo-Irie, M., Irie, M., Nakazawa, T., Mohri, H., Morphological changes in eupyrene and apyrene spermatozoa in the reproductive tract of the male butterfly Atrophaneura alcinous Klug (1998) Invert. Reprod. Dev., 34, pp. 259-268
dc.descriptionLai-Fook, J., Structural comparison between eupyrene and apyrene spermiogenesis in Calpodes ethlius (Hesperiidae: Lepidoptera) (1982) Can. J. Zool., 60, pp. 1216-1230
dc.descriptionL'Hernault, S.W., Rosenbaum, J.L., Chlamydomonas alpha-tubulin is posttranslationally modified by acetilation on the Sigma-amino group of a lysine (1985) Biochemistry, 24, pp. 473-478
dc.descriptionLi, Q., Joshi, H.C., Gamma tubulin is a minus end-specific microtubule binding protein (1995) J. Cell Biol., 131, pp. 207-214
dc.descriptionLiang, A., Ruiz, F., Heckmann, K., Klotz, C., Tollon, Y., Beisson, J., Wright, M., Gamma tubulin is permanently associated with basal bodies in ciliates (1996) Eur. J. Cell Biol., 70, pp. 331-338
dc.descriptionLopes, L.C., Ribeiro, K.C., Benchimol, M., Immunolocalization of tubulin isoforms and post-translational modification in the protists Tritrichomonas foetus and Trichomonas vaginalis (2001) Histochem. Cell Biol., 116, pp. 17-29
dc.descriptionLudueña, R.F., Multiple forms of tubulin: Different gene products and covalent modifications (1998) Int. Rev. Cytol., 178, pp. 207-275
dc.descriptionMacRae, T.H., Tubulin post-translational modifications. Enzymes and their mechanisms of action (1997) Eur. J. Biochem., 244, pp. 265-278
dc.descriptionMancini, K., Dolder, H., Ultrastructure of apyrene and eupyrene spermatozoa from the seminal vesicle of Euptoieta hegesia (Lepidoptera: Nymphalidae) (2001) Tissue Cell., 33, pp. 301-308
dc.descriptionMancini, K., Dolder, H., Sperm morphology and arrangement along the male reproductive tract of the butterfly Euptoieta hegesia (Insecta: Lepidoptera) (2003) Invert. Reprod. Dev., 44, pp. 107-117
dc.descriptionMancini, K., Dolder, H., Dichotomic spermiogenesis of Euptoieta hegesia (Lepidoptera: Nymphalidae) (2004) Braz. J. Morphol. Sci., 21, pp. 13-23
dc.descriptionMancini, K., Dolder, H., Protein detection in spermatids and spermatozoa of the butterfly Euptoieta hegesia (Lepidoptera) (2004) Biocell, 28, pp. 299-310
dc.descriptionMencarelli, C., Bré, M., Levilliers, N., Dallai, R., Accessory tubules and axonemal microtubules of Apis mellifera sperm flagellum differ in their tubulin isoform content (2000) Cell Motil. Cytoskel., 47, pp. 1-12
dc.descriptionMultigner, L., Pignot-Paintrand, I., Saoudi, Y., Job, D., Plessmann, U., Rüdiger, M., Weber, K., The A and B tubules of the outer doublets of sea urchin sperm axonemes are composed of different tubulin variants (1996) Biochemistry, 35, pp. 10862-10871
dc.descriptionOakley, C.E., Oakley, B.R., Identification of gamma-tubulin, a new member of the tubulin superfamily encoded by mipA gene in Aspergillus nidulans (1989) Nature, 338, pp. 662-664
dc.descriptionOakley, B.R., Oakley, C.E., Ion, Y., Jung, M.K., Gamma tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans (1990) Cell, 61, pp. 1289-1301
dc.descriptionPhillips, D.M., Insect sperm: Their structure and morphogenesis (1970) J. Cell Biol., 44, pp. 243-277
dc.descriptionPhillips, D.M., Morphogenesis of the lacinate appendages of lepidopteran spermatozoa (1971) J. Ultrastruct. Res., 34, pp. 567-585
dc.descriptionRiemann, J.G., Metamorphosis of sperm of the cabbage lopper Trichoplusia ni during passage from the testes to the female spermatheca (1970) Comparative Spermatology, pp. 321-331. , B. Baccetti (Ed.). Academic Press New York
dc.descriptionRiemann, J.G., Thorson, B.J., Sperm maturation in the male and female genital tracts of Anagasta kuhniella (Lepidoptera: Pyralididae) (1971) Int. J. Insect Morphol. Embryol., 1, pp. 11-19
dc.descriptionSasse, R., Gull, K., Tubulin post-translational modifications and the construction of microtubular organelles in Trypanosoma brunei (1988) Cell Sci., 90, pp. 577-589
dc.descriptionSchulze, E., Asai, D.J., Bulinski, J.C., Kirschner, M., Post-translational modification and microtubule stability (1987) J. Cell Biol., 105, pp. 2167-2177
dc.descriptionShu, H.-B., Joshi, H.C., Gamma-tubulin can both nucleate microtubule assembly and self-assemble into novel tubular structures in mammalian cells (1995) J. Cell Biol., 130, pp. 1137-1147
dc.descriptionSilberglied, R.E., Shepherd, J.G., Dickinson, J.L., Eunuchs: The role of apyrene sperm in lepidoptera? (1984) Am. Nat., 123, pp. 255-265
dc.descriptionSnook, R.R., Is the production of multiple sperm types adaptive? (1997) Evolution, 51, pp. 797-808
dc.descriptionSnook, R.R., The risk of sperm competition and the evolution of sperm heteromorphism (1998) Ann. Behav., 56, pp. 1497-1507
dc.descriptionTaddei, A.R., Gambellini, G., Fausto, A.M., Baldacci, A., Mazzini, M., Immunolocalization of different tubulin epitopes in the spermatozoon of Bacillus rossius (Insecta, Phasmatodea) (2000) J. Submicrosc. Cytol. Pathol., 32, pp. 635-643
dc.descriptionTakemura, R., Okabe, S., Umeyama, T., Kanai, Y., Cowan, N., Hirokawa, N., Increased microtubule-stability and alpha-tubulin acetylation in cells transfected with microtubule-associated proteins MAP1B, MAP2 or tau (1992) J. Cell Sci., 103, pp. 953-964
dc.descriptionWebster, D.L., Borisy, G.G., Microtubules are acetylated in domains that turnover slowly (1989) J. Cell Sci., 92, pp. 57-65
dc.descriptionWilson, P.J., Forer, A., Acetylated alpha-tubulin in spermatogenic cells of the crane fly Nephrotoma suturalis: Kinetochore microtubules are selectively acetylated (1989) Cell Motil. Cytoskel., 14, pp. 237-250
dc.descriptionWolf, K.W., Spindle membranes and microtubules are coordinately reduced in apyrene relative to eupyrene spermatocyte of Inachisio (Lepidoptera: Nymphalidae) (1992) J. Submicrosc. Cytol. Pathol., 24, pp. 381-394
dc.descriptionWolf, K.W., Cytology of lepidoptera. VIII. Acetylation of alpha-tubulin in mitotic and meiotic spindles of two Lepidoptera species, Ephestia kuehniella (Pyralidae) and Pieris brassicae (Pieridae) (1996) Protoplasma, 190, pp. 88-98
dc.descriptionWolf, K.W., Immunocytochemical evidence of a tubulin reserve at the tip of growing flagella in spermatogenesis of the Mediterranean Mealmoth, Ephestia kuehniella Z. (Pyralidae, Lepidoptera, Insecta) (1996) Acta Zool. (Stockholm), 77, pp. 79-84
dc.descriptionWolf, K.W., Centrosome structure is very similar in eupyrene and apyrene spermatocyte of Ephestia kuehniella (Pyralidae: Lepidoptera: Insecta) (1997) Invert. Reprod. Dev., 31, pp. 39-46
dc.descriptionWolf, K.W., Baumgart, K., Traut, W., Cytology of lepidoptera. II. Fine structure of eupyrene and apyrene primary spermatocytes in Orgyia thyellina (1988) Eur. J. Cell Biol., 44, pp. 57-67
dc.descriptionWolf, K.W., Bastmeyer, M., Cytology of lepidoptera. V. The microtubule cytoskeleton in eupyrene spermatocyte of Ephestia kuehniella (Pyralidae), Inachisio (Nymphalidae) and Orgyia antiqua (Lymantriidae) (1991) Eur. J. Cell Biol., 55, pp. 225-237
dc.descriptionWolf, K.W., Bastmeyer, M., Cytology of lepidoptera. VI. Immunolocalization of microtubules in detergent-extracted apyrene spermatocytes of Kuehniella Z (1991) Eur. J. Cell Biol., 55, pp. 238-247
dc.descriptionWolf, K.W., Joshi, H.C., Microtubule organization and distribution of gamma-tubulin in male meiosis of Lepidoptera (1996) Mol. Reprod. Dev., 45, pp. 547-559
dc.languageen
dc.publisher
dc.relationTissue and Cell
dc.rightsfechado
dc.sourceScopus
dc.titleImmunocytochemical Localization Of Tubulins In Spermatids And Spermatozoa Of Euptoieta Hegesia (lepidoptera: Nymphalidae)
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución