Tnfα Contributes For Attenuating Both Y397fak And Y416src Phosphorylations In Osteoblasts

dc.creatorCavagis A.D.M.
dc.creatorTakamori E.R.
dc.creatorGranjeiro J.M.
dc.creatorOliveira R.C.
dc.creatorFerreira C.V.
dc.creatorPeppelenbosch M.P.
dc.creatorZambuzzi W.F.
dc.date2014
dc.date2015-06-25T17:56:49Z
dc.date2015-11-26T14:47:09Z
dc.date2015-06-25T17:56:49Z
dc.date2015-11-26T14:47:09Z
dc.date.accessioned2018-03-28T21:57:21Z
dc.date.available2018-03-28T21:57:21Z
dc.identifier
dc.identifierOral Diseases. Blackwell Publishing Ltd, v. 20, n. 8, p. 780 - 786, 2014.
dc.identifier1354523X
dc.identifier10.1111/odi.12202
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84911990471&partnerID=40&md5=c3c967b0ddefc3886a74d623293b612c
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/87123
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/87123
dc.identifier2-s2.0-84911990471
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1253150
dc.descriptionObjective: Our poor understanding of how inflammatory mediators can affect osteoblast behavior led us to investigate the tumor necrosis factor (TNF)α-induced focal adhesion kinase (FAK) and Src phosphorylation. Material and Methods: MC3T3-E1 pre-osteoblast cells were harvested at specific time points after either TNFα treatment or RAW267 stimulated conditioned medium, and thereafter cell extracts were prepared for Immunoblotting assay. ELISA detected TNFα content at conditioned medium. Tumor necrosis factor-α-neutralizing antibodies also were used. Results: It was possible to show that TNFα provokes attenuation at Y-phosphorylation of both FAK (at Y397) and Src (at Y416) proteins (P < 0.05), suggesting a decrease in their activities. The very similar profile was observed when osteoblasts were incubated with conditioned medium from lipopolysaccharide (LPS)-stimulated macrophages, it being significantly different than control (FAK and Src, P < 0.05). Nevertheless, in order to validate these findings, we decided to pre-incubate osteoblasts with anti-TNFα neutralizing antibody (2 μg ml-1) prior exposing to conditioned medium. Importantly, our results revealed that there was a diminution on those conditioned medium effects when the same biological parameters were evaluated (P < 0.05). Moreover, we also showed that TNFα impairs osteoblast adhesion, suggesting an interesting role on osteoblast performance. Conclusions: Altogether, these results suggest that LPS-stimulated macrophage mediators attenuate both FAK and Src activations in osteoblast, suggesting a novel role for TNFα on osteoblast performance.
dc.description20
dc.description8
dc.description780
dc.description786
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dc.languageen
dc.publisherBlackwell Publishing Ltd
dc.relationOral Diseases
dc.rightsfechado
dc.sourceScopus
dc.titleTnfα Contributes For Attenuating Both Y397fak And Y416src Phosphorylations In Osteoblasts
dc.typeArtículos de revistas


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