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An alternative easy method for antibody purification and analysis of protein-protein interaction-using GST fusion proteins immobilized onto glutathione-agarose
(Springer, 2014-01)
Immobilization of small proteins designed to perform protein-protein assays can be a difficult task. Often, the modification of reactive residues necessary for the interaction between the immobilized protein and the matrix ...
Apoptose em otorrinolaringologia e cabeça e pescoço
(2003-06-01)
This paper intend to review recent advances in our understanding of programmed cell death, or apoptosis, and discuss implications of these basic science advances in the development of causes and potential treatments of a ...
H-bond refinement for electron transfer membrane-bound protein-protein complexes: cytochrome c oxidase and cytochrome c552
(Elsevier, 2013-06)
In this study we propose a protocol to evaluate membrane-bound cytochrome c oxidase–cytochrome c552 docking candidates. An initial rigid docking algorithm generates docking poses of the cytochrome c oxidase–cytochrome c552, ...
The effects of copper ions on the synthesis of periplasmic and membrane proteins in Acidithiobaeillus ferrooxidans as analyzed by SDS-PAGE and 2D-PAGE
(Elsevier B.V., 2003-10-01)
The effects of 200 mM copper ions on the synthesis of membrane and periplasmic proteins were investigated in iron-grown cells of Acidithiobacillus ferrooxidans (At. ferrooxidans). Total membrane protein profiles of cells ...
Amaranth protein films prepared with high-pressure treated proteins
(Elsevier, 2015-01)
This work studies the effect of using high-pressure modified amaranth proteins in the preparation of edible film and compares the efficiency of high pressure and thermal treatment on the functionality of amaranth protein ...