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LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
(Wiley VCH Verlag, 2009-12)
The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein ...
Regulating the regulators: Serine/arginine-rich proteins under scrutiny
(John Wiley & Sons Inc, 2012-10)
Serine/arginine-rich (SR) proteins are among the most studied splicing regulators. They constitute a family of evolutionarily conserved proteins that, apart from their initially identified and deeply studied role in splicing ...
Engineering of the baculovirus expression system for optimized protein production
(Springer, 2019-01)
Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multipleinsect pests. In addition to their application in pest control, baculoviruses have become a ...
Computational prediction of short linear motifs from protein sequences
(Springer, 2015-01)
Short Linear Motifs (SLiMs) are functional protein microdomains that typically mediate interactions between a short linear region in one protein and a globular domain in another. SLiMs usually occur in structurally disordered ...
Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
(Public Library of Science, 2016-08)
Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 ...
Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
(Bioscientifica, 2013-02-11)
Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite ...
Purification of Recombinant Galectins Expressed in Bacteria
(Cell Press, 2020-12)
Galectins are soluble lectins that participate in many physiological and pathological functions. Since they can act extracellularly, the use of the recombinant protein is a recurrent strategy for studying their biological ...
Daily expression of sodium-dependent glucose cotransporter-1protein in jejunum during rat ontogeny
(Elsevier, 2019-09)
It is widely known that intestinal capacities such as the enzymatic hydrolysis of carbohydrates, lipids and proteins, and the subsequent absorption of the hydrolyzed products, are evolutionary matched to dietary loads and ...
Functional interconnections of Arabidopsis exon junction complex proteins and genes at multiple steps of gene expression
(Oxford University Press, 2011-10)
The exon junction complex (EJC) is deposited on mRNA after splicing and participates in several aspects of RNA metabolism, from intracellular transport to translation. In this work, the functional and molecular interactions ...
Bone Morphogenetic Proteins (BMPs) in the bovine oviduct: Differential expression of BMP-5 in the isthmus during the estrous cycle
(Elsevier, 2014-05)
Bone morphogenetic proteins (BMPs) play a crucial role in mammalian reproduction, but little is known about their expression and function in the oviduct, where preimplantation events take place. In the present study, ...