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Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
(Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária, 2013-02-01)
O presente estudo objetivou avaliar uma técnica de nested PCR espécie-específica delineada a partir de PCR espécie-específica descrita anteriormente para detecção de B. ovis em sêmen e urina de carneiros infectados ...
Malaria diagnosis from pooled blood samples: comparative analysis of real-time PCR, nested PCR and immunoassay as a platform for the molecular and serological diagnosis of malaria on a large-scale
(Instituto Oswaldo Cruz, Ministério da Saúde, 2011)
Malaria diagnoses has traditionally been made using thick blood smears, but more sensitive and faster techniques are required to process large numbers of samples in clinical and epidemiological studies and in blood donor ...
A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
(Blackwell Publishing, 2004-01-01)
Aims: Detection of Xylella fastidiosa in citrus plants and insect vectors.Methods and Results: Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp ...
Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples
(2022-01-01)
The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from ...
Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems
(Sociedade Brasileira de Microbiologia, 2008-09-01)
A Nested-PCR (N-PCR) tem como objetivo melhorar a sensibilidade do diagnóstico direto da Pneumonia Enzoótica Suína, pois o isolamento do Mycoplasma hyopneumoniae é trabalhoso tornando-se inviável na rotina. Neste trabalho, ...
Canine ehrlichiosis: clinical, hematological, serological and molecular aspects
(Universidade Federal de Santa Maria (UFSM), 2008-06-01)
O objetivo deste estudo foi comparar técnicas para detecção direta de Ehrlichia canis (detecção de mórulas em esfregaço sangüíneo e nested PCR), testes sorológicos (Dot-ELISA e Reação de Imunofluorescência Indireta - RIFI) ...
Development and validation of nested-PCR for the diagnosis of clinical and subclinical infectious laryngotracheitis
(ELSEVIER SCIENCE BV, 2008)
A standardised nested-PCR method that amplifies a region of the glycoprotein E gene of avian infectious laryngotracheitis virus (ILTV) has been developed for the diagnosis of infection by Gallid herpesvirus 1. The two sets ...
Avaliação das técnicas de RT-PCR e heminested RT-PCR em cérebros de cães com sinais neurológicos compatíveis com cinomose
(2015-01-01)
The diagnostic value of RT-PCR and hemi-nested RT-PCR (hnRT-PCR) was compared in brain samples of dogs presenting neurological signs compatible with canine distemper. Samples of central nervous system (CNS) were collected ...