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Electroporation of postimplantation mouse embryos in utero
(Cold Spring Harbor Laboratory Press, 2018-02)
Gene transfer by electroporation is possible in mouse fetuses within the uterus. As described in this protocol, the pregnant female is anesthetized, the abdominal cavity is opened, and the uterus with the fetuses is ...
Cholesterol Depletion Disorganizes Oocyte Membrane Rafts Altering Mouse Fertilization
(Public Library of Science, 2013-04-25)
Drastic membrane reorganization occurs when mammalian sperm binds to and fuses with the oocyte membrane. Two oocyte protein families are essential for fertilization, tetraspanins and glycosylphosphatidylinositol-anchored ...
An efficient experimental strategy for mouse embryonic stem cell differentiation and separation of a cytokeratin-19-positive population of insulin-producing cells
(Wiley Blackwell Publishing, Inc, 2008-07-08)
Embryonic stem cells are a potential source for insulin‐producing cells, but existing differentiation protocols are of limited efficiency. Here, the aim has been to develop a new one, which drives development of embryonic ...
The autoimmune response elicited by mouse hepatitis virus (mhv-a59) infection is modulated by liver tryptophan-2,3- dioxygenase (tdo)
(Elsevier Science, 2019-11)
In a previous work we demonstrated that inhibition of mouse indoleamine 2,3-dioxygenase (IDO) by methyltryptophan (MT) exacerbated the pathological actions of mouse hepatitis virus (MHV-A59) infection, suggesting that ...
Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
(Elsevier Inc, 2010-05-01)
Mouse oocytes acquire the ability to replicate DNA during meiotic maturation, presumably to ensure that DNA replication does not occur precociously between MI and MII and only after fertilization. Acquisition of DNA ...
Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
(Cell Press, 2018-04)
Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst ...
Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secret their content
(Company of Biologists, 2018-10)
Oocyte in vitro maturation does not entirely support all the nuclear and cytoplasmic changes that occur physiologically and it is poorly understood whether in vitro maturation affects the competence of cortical granules ...
Induction of redox changes, inducible nitric oxide synthase and cyclooxygenase-2 by chronic cadmium exposure in mouse peritoneal macrophages
(Elsevier Ireland, 2003-11-30)
Redox changes and the secretion of inflammatory mediators were investigated in resident peritoneal macrophages of mice chronically exposed to cadmium (Cd, 15 ppm for 2 months) through drinking water. Our results showed ...
Progesterone and Allopregnanolone Neuroprotective Effects in the Wobbler Mouse Model of Amyotrophic Lateral Sclerosis
(Springer/Plenum Publishers, 2021-06)
Progesterone regulates a number of processes in neurons and glial cells not directly involved in reproduction or sex behavior. Several neuroprotective effects are better observed under pathological conditions, as shown in ...
Impaired brain glucose metabolism and presynaptic dopaminergic functioning in a mouse model of schizophrenia
(Springer, 2020-04)
Background: Schizophrenia is a disease diagnosed by visible signs and symptoms from late adolescence to early adulthood. The etiology of this disease remains unknown. An objective diagnostic approach is required. Here, we ...