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A Smartphone-Based Low-Cost Inverted Laser Fluorescence Microscope for Disease Diagnosis
(MDPI, 2022)
Fluorescence microscopy is an important tool for disease diagnosis, often requiring costly optical components, such as fluorescence filter cubes and high-power light sources. Due to its high cost, conventional fluorescence ...
Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
(Wiley VCH Verlag, 2008-01)
We have designed and synthesized a new functional (amino reactive)highly efficient fluorescent molecular switch (FMS) with a photochromicdiarylethene and a rhodamine fluorescent dye. The reactive group in thisFMS ...
Fluorescence sensors for imaging membrane lipid domains and cholesterol
(Elsevier Academic Press Inc., 2021-10)
Lipid membrane domains are supramolecular lateral heterogeneities of biological membranes. Of nanoscopic dimensions, they constitute specialized hubs used by the cell as transient signaling platforms for a great variety ...
Compact and reflective light-sheet microscopy for long-term imaging of living embryos
(Optical Society of America, 2020-05)
The development of light-sheet fluorescence microscopy has been a revolution for developmental biology as it allows long-term imaging during embryonic development. An important reason behind the quick adoption has been the ...
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
(Institution of Russian Academy of Sciences, 2021-02)
The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such ...
Mapping pixel dissimilarity in wide-field super-resolution fluorescence microscopy
(American Chemical Society, 2015-05)
Recent advances in fluorescence bioimaging with single-molecule sensitivity have relied on the analysis and visualization of single-molecule data obtained on smart fluorophores. We describe an alternative method to enhance ...
Quantitative expansion microscopy for the characterization of the spectrin periodic skeleton of axons using fluorescence microscopy
(Nature Publishing Group, 2020-02)
Fluorescent nanoscopy approaches have been used to characterize the periodic organization of actin,spectrin and associated proteins in neuronal axons and dendrites. This membrane-associated periodicskeleton (MPS) is conserved ...
Exploring the Dynamics of Cell Processes through Simulations of Fluorescence Microscopy Experiments
(Cell Press, 2015-06)
Fluorescence correlation spectroscopy (FCS) methods are powerful tools for unveiling the dynamical organization of cells. For simple cases, such as molecules passively moving in a homogeneous media, FCS analysis yields ...
Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
(Wiley VCH Verlag, 2008-02)
During the last decade far-field fluorescence microscopy methodshave evolved that have resolution far below the wavelength oflight. To outperform the limiting role of diffraction, all thesemethods, in one way or another, ...