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Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture
(2014)
Mammalian cells are the most frequently used hosts for biopharmaceutical proteins manufacturing. Inoculum quality is a key element for establishing an efficient bioconversion process. The main objective in inoculation ...
The tyrosine kinase inhibitor nilotinib is more efficient than mitotane in decreasing cell viability in spheroids prepared from adrenocortical carcinoma cells
(BioMed Central, 2018)
Abstract
Background
New drugs for adrenocortical carcinoma (ACC) are needed because most patients undergo rapid disease progression despite surgery and adjuvant therapy ...
Dipteran chromosomes: a simple method for obtaining high quality chromosomal preparations
(Indian Academy of Sciences, 2014-12)
For cytogenetic studies it is necessary to develop an efficient method of chromosome preparation with wellpreserved and dispersed chromosomes and little or no cell-wall debris. In this study, we describe a method for ...
Natural killer cells as helper cells in dendritic cell cancer vaccines
(Frontiers Research Foundation, 2015-01)
Vaccine-based cancer immunotherapy has generated highly variable clinical results due to differing methods of vaccine preparation and variation in patient populations among other lesser factors. Moreover, these clinical ...
Compact TiO2 blocking-layer prepared by LbL for perovskite solar cells
(2021-01-15)
Blocking-layer are very important for the overall efficiency improvement in perovskite solar cells, PSCs. This layer must be thin enough to allow the light to reach the perovskite layer and have low electrical resistance ...
New protocol for cell culture to obtain mitotic chromosomes in fishes
(2018-01-01)
Cell culture is an excellent alternative for the maintenance of cell lines and to obtain quality chromosome preparations of fishes. However, this methodology is still little employed, mainly because of the difficulty of ...
Cytotoxicity of resin-based luting cements to pulp cells
(2014)
To evaluate the cytotoxicity of components released from different types of luting cements to two cell lines obtained from pulp tissue. Methods: Three types of luting cements were evaluated, distributed into the following ...
A systematic study of transfection efficiency and cytotoxicity in HeLa cells using iron oxide nanoparticles prepared with organic and inorganic bases
(ELSEVIER SCIENCE BVAMSTERDAM, 2012)
Magnetic iron oxide nanoparticles (magnetite) (MNPs) were prepared using different organic and inorganic bases. Strong inorganic base (KOH) and organic bases (NH4OH and 1,4-diazabicyclo[2.2.2]octane (DABCO)) were used in ...
A systematic study of transfection efficiency and cytotoxicity in HeLa cells using iron oxide nanoparticles prepared with organic and inorganic bases
(Elsevier B.V., 2012-12-01)
Magnetic iron oxide nanoparticles (magnetite) (MNPs) were prepared using different organic and inorganic bases. Strong inorganic base (KOH) and organic bases (NH4OH and 1,4-diazabicyclo[2.2.2]octane (DABCO)) were used in ...