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Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins
(2015-06)
The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RPHPLC,
SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified
or assigned to families. ...
Danger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis
(2015-12-01)
Four specimens of the olive sea snake, Aipysurus laevis, were collected off the coast of Western Australia, and the venom proteome was characterized and quantitatively estimated by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF ...
Proteomic analysis of Bothrops pirajai snake venom and characterization of BpirMP, a new P-I metalloproteinase
(2013-03-27)
Bothrops pirajai snake venom was analyzed by a proteomic strategy. Proteins were separated by RP-HPLC, followed by SDS-PAGE, in-gel tryptic digestion, identification by MALDI-TOF/TOF mass spectrometry, and assignment to ...
Integrative characterization of the venom of the coral snake Micrurus dumerilii (Elapidae) from Colombia: proteome, toxicity, and cross-neutralization by antivenom
(2016-03)
In Colombia, nearly 2.8% of the 4200 snakebite accidents recorded annually are inflicted by coral snakes (genus
Micrurus). Micrurus dumerilii has a broad distribution in this country, especially in densely populated ...
An untargeted metabolomic assessment of cocoa beans during fermentation
(2016-11)
Fermentation is a critical step in the processing of high quality cocoa; however, the biochemistry behind is still not well understood at a molecular level. In this research, using a non-targeted approach, the main metabolomic ...
Structural and functional properties of BaTX, a new Lys49 phospholipase A2 homologue isolated from the venom of the snake Bothrops alternatus
(2007-04)
BaTX PLA2, a K49 phospholipase A2 homologue was purified from Bothrops alternatus venom after two chromatographic steps, molecular exclusion on Superdex 75 and reverse phase HPLC on μ-Bondapack C-18. A molecular mass of ...
Screening for target toxins of the antiophidic protein DM64 through a gel-based interactomics approach
(2017-01)
DM64 is a glycosylated protein with antivenomactivity isolated fromthe serum of the opossumDidelphis aurita.
It binds non-covalently to myotoxins I (Asp49) and II (Lys49) from Bothrops asper venom and inhibits their
myotoxic ...
Comparison of venom composition and biological activities of the subspecies Crotalus lepidus lepidus, Crotalus lepidus klauberi and Crotalus lepidus morulus from Mexico
(2013-08-01)
The rock rattlesnakes Crotalus lepidus comprise a group (lepidus, klauberi, morulus and
maculosus) of poorly known mountain cold-tolerant snakes in Mexico. In particular, Crotalus
lepidus morulus is a snake endemic of ...
Biological and structural characterization of crotoxin and new isoform of crotoxin B PLA2 (F6a) from Crotalus durissus collilineatus snake venom
(2006)
A new crotoxin B isoform PLA2 (F6a), from Crotalus durissus collilineatus was purified from
by one step reverse phase HPLC chromatography using l-Bondapack C-18 column analytic.
The new crotoxin B isoform PLA2 (F6a), ...
Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum
(2002-12-11)
Bothrops snake venoms are known to induce local tissue damage such as hemorrhage and myonecrosis. The opossum Didelphis marsupialis is resistant to these snake venoms and has natural venom inhibitors in its plasma. The aim ...