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Free-floating adult human brain-derived slice cultures as a model to study the neuronal impact of Alzheimer's disease-associated A beta oligomers
(Elsevier B.V., 2018-09-01)
Background: Slice cultures have been prepared from several organs. With respect to the brain, advantages of slice cultures over dissociated cell cultures include maintenance of the cytoarchitecture and neuronal connectivity. ...
Human breast tumor slices as an alternative approach to cell lines to individualize research for each patient
(Lippincott Williams & Wilkins, 2012-07-01)
There are several breast cancer experimental models including cell lines, which are commonly used due to ease of handling and storage. However, the continued propagation of cell lines and distribution among laboratories ...
Evaluation of follicular growth and tissue viability in vitrified/warmed domestic dog ovaries after in vitro culture
(2017-04-01)
Cryopreservation of gametes is an important tool to preserve fertility, but for most species, including domestic dogs, data regarding ovarian tissue cryopreservation are limited. We aimed to evaluate the follicular and ...
Brain stem slice conditioned medium contains endogenous BDNF and GDNF that affect neural crest boundary cap cells in co-culture
(Elsevier, 2014-04)
Conditioned medium (CM), made by collecting medium after a few days in cell culture and then re-using it to further stimulate other cells, is a known experimental concept since the 1950s. Our group has explored this technique ...
Short-Term Free-Floating Slice Cultures from the Adult Human Brain
(Journal Of Visualized Experiments, 2019-11-01)
Organotypic, or slice cultures, have been widely employed to model aspects of the central nervous system functioning in vitro. Despite the potential of slice cultures in neuroscience, studies using adult nervous tissue to ...
Human breast tumor slices: A model for identification of vitamin D regulated genes in the tumor microenvironment
(PERGAMON-ELSEVIER SCIENCE LTD, 2010)
While many studies have addressed the direct effects of 1 alpha,25(OH)(2)D(3) on breast cancer (BC) cells, stromal-epithelial interactions, which are important for the tumor development, have been largely ignored. In ...
Further evidence for the neuroplastic role of cannabinoids: a study in organotypic hippocampal slice cultures
(Springer, 2015-08)
Endocannabinoid receptors CB1R and CB2R are present in the CNS and modulate synaptic activity. By using an in vitro model, two concentrations of CB1R agonist ACEA at 0.5 and 5 μM doses and CB1R antagonist AM251 at 1 and ...
Gene expression and enzyme function of two cytochrome P450 3A isoenzymes in rat and cattle precision cut liver slices
(Taylor & Francis Ltd, 2015-01)
1. Precision-cut liver slices are one of the in vitro models used in studies concerning xenobiotic metabolism. Sparse information on this field is actually available for cattle and other veterinary species. 2. The aim ...
Neuroprotection by steroids after neurotrauma in organotypic spinal cord cultures: A key role for progesterone receptors and steroidal modulators of GABAA receptors
(Elsevier, 2013-08-31)
Progesterone is neuroprotective after spinal cord injury, however its mechanism of action remains unexplored. Here we used organotypic spinal cord slice cultures from 3 weeks-old mice to evaluate the mechanisms of ...
Cdk5 is essential for amphetamine to increase dendritic spine density in hippocampal pyramidal neurons
(Frontiers Research Foundation, 2017-11)
Psychostimulant drugs of abuse increase dendritic spine density in reward centers of the brain. However, little is known about their effects in the hippocampus, where activity-dependent changes in the density of dendritic ...