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Recent advancements of EC-QCL based mid-IR transmission spectroscopy of proteins and application to analysis of bovine milk
(IOS Press, 2018-01)
Background:High emission powers of external cavity-quantum cascade laser (EC-QCL) light sources allow to employ significantly larger path lengths for infrared (IR) transmission measurements compared to conventional ...
Structural and antioxidant properties of cowpea protein hydrolysates
(Elsevier, 2021-06)
Cowpea protein isolates extracted at pH 8.0 (I8) and pH 10.0 (I10) were hydrolysed by alcalase to obtain limited (I8LH and I10LH) and extensive (I8HH and I10HH) hydrolysates. Hydrolysis degree was 2.3% for I8LH, 4.6% for ...
Aromatic clusters in protein-protein and protein-drug complexes
(BioMed Central, 2020-05)
Aromatic rings are important residues for biological interactions and appear to a large extent as part of protein-drug and protein-protein interactions. They are relevant for both protein stability and molecular recognition ...
EC-QCL mid-IR transmission spectroscopy for monitoring dynamic changes of protein secondary structure in aqueous solution on the example of β-aggregation in alcohol-denaturated α-chymotrypsin
(Springer Heidelberg, 2016-06)
In this work, a novel EC-QCL-based setup for mid-IR transmission measurements in the amide I region is introduced for monitoring dynamic changes in secondary structure of proteins. For this purpose, α-chymotrypsin (aCT) ...
External cavity-quantum cascade laser infrared spectroscopy for secondary structure analysis of proteins at low concentrations
(Nature Publishing Group, 2016-09)
Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy are analytical techniques employed for the analysis of protein secondary structure. The use of CD spectroscopy is limited to low protein concentrations ...
FTIR Spectroscopy Structural Analysis of the Interaction between Lactobacillus kefir S-layers and Metal Ions
(Elsevier Science, 2011-02)
FTIR spectroscopy was used to structurally characterize the interaction of S-layer proteins extracted from two strains of Lactobacillus kefir (the aggregating CIDCA 8348 and the non-aggregating JCM 5818) with metal ions ...
Characterization of S-layer proteins of Lactobacillus by FTIR spectroscopy and differential scanning calorimetry
(Elsevier Science, 2009-05)
FTIR spectroscopy was used for the characterization of S-layer proteins extracted from microorganisms isolated from kefir grains. S-layer from Lactobacillus brevis ATCC 8287 has been already characterized [G. Vidgren, I. ...
A novel enolase from Taenia solium metacestodes and its evaluation as an immunodiagnostic antigen for porcine cysticercosis
(Elsevier, 2018)
Cysticercosis is a worldwide parasitic disease of humans and pigs principally caused by infection with the larvae of the pork tapeworm Taenia solium. Through the use of the recently-made-available T. solium genome, we ...
NetSurfP-2.0: Improved prediction of protein structural features by integrated deep learning
(Veterinary and Human Toxicology, 2019-06-20)
The ability to predict local structural features of a protein from the primary sequence is of paramount importance for unraveling its function in absence of experimental structural information. Two main factors affect the ...