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Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
(American Scientific Publishers, 2012-10)
The reagent MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole) is reduced to formazan in living cells, giving a purple colored product. The product absorbance issusceptible to be measured ...
Tetrazolium salts and formazan products in Cell Biology: Viability assessment, fluorescence imaging, and labeling perspectives
(Elsevier Gmbh, Urban & Fischer Verlag, 2018-04)
For many years various tetrazolium salts and their formazan products have been employed in histochemistry and for assessing cell viability. For the latter application, the most widely used are 3-(4,5-dimethyl-2-thiazolyl ...
High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells
(Springer Heidelberg, 2017-08-29)
The aim of this study was to investigate the cytotoxic and genotoxic effects of high copper (Cu) concentrations on bovine cumulus cells (CCs) cultured in vitro. We evaluated the effect of 0, 120, 240, and 360 μg/dL Cu added ...
Doramectin induced cytotoxic and genotoxic effects on bovine peripheral lymphocytes and cumulus cells in vitro
(Taylor & Francis, 2019-02)
The effect of doramectin (DOR) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PL) and cumulus cells (CC). The cytotoxicity and genotoxicity of DOR were assessed using 3-(4,5-dimethy ...
Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
(Academic Press Ltd - Elsevier Science Ltd, 2017-05-15)
The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM, ...
Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles
(Wiley Blackwell Publishing, Inc, 2018-11)
Riboflavin (Rf) is an endogenous photosensitizer, which can participate in Type I and Type II processes. We have recently shown that the yield of the triplet excited states of Rf is enhanced in the presence of pectin-coated ...
Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro: peripheral lymphocytes and cumulus cells
(Springer Heidelberg, 2019-01)
The in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic ...