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Expression of exogenous genes in Trypanosoma cruzi: Improving vectors and electroporation protocols
(Springer, 2004-02)
To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol and expression vectors which use luciferase and green and red fluorescent proteins as reporter genes. In transient ...
Codon optimization of bacterial luciferase (lux) for expression in mammalian cells
(Springer Heidelberg, 2005)
Enhancement of nucleic acid delivery to hard-to-transfect human colorectal cancer cells by magnetofection at laminin coated substrates and promotion of the endosomal/lysosomal escape
(Royal Society of Chemistry, 2015)
Despite a great diversity of nanomaterials, such as cationic lipid, polymers or inorganic nanoparticles, has been developed in order to carry nucleic acids across plasma membrane, these methodologies have still insufficient ...
Relevance of the N-terminal and major hydrophobic domains of non-structural protein 3A in the replicative process of a DNA-launched foot-and-mouth disease virus replicon
(Springer Wien, 2018-07)
A foot-and-mouth disease virus (FMDV) DNA-launched reporter replicon containing a luciferase gene was used to assess the impact of non-structural (NS) protein 3A on viral replication. Independent deletions within the ...
Translation fidelity coevolves with longevity
(Wiley Blackwell Publishing, Inc, 2017-10)
Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis ...
siRNA modified with 2′-deoxy-2′-c-methylpyrimidine nucleosides
(Wiley VCH Verlag, 2018-03)
(2′S)-2′-Deoxy-2′-C-methyluridine and (2′R)-2′-deoxy-2′-C-meth-yluridine were incorporated in the 3’-overhang region of the sense and antisense strands and in positions 2 and 5 of the seed region of siRNA duplexes directed ...
Reconstructing the evolutionary history of F420-dependent dehydrogenases
(Nature Publishing Group, 2018-12)
During the last decade the number of characterized F420-dependent enzymes has significantly increased. Many of these deazaflavoproteins share a TIM-barrel fold and are structurally related to FMN-dependent luciferases and ...
Establishment and characterization of a Madin-Darby canine kidney reporter cell line for influenza A virus assays
(American Society for Microbiology, 2010-07)
Influenza virus diagnosis has traditionally relied on virus isolation in chicken embryo or cell cultures. Many laboratories have adopted rapid molecular methods for detection of influenza viruses and discontinued routine ...
Aquaporin-2 Promoter Is Synergistically Regulated by Nitric Oxide and Nuclear Factor of Activated T Cells.
(Karger, 2011-10)
Background/aims: We have previously shown that aquaporin-2 (AQP2) is down-regulated in the renal medulla of rats made hypertensive by chronic inhibition of nitric oxide synthase (NOS). It has been shown that AQP2 expression ...
Role of CREB on heme oxygenase-1 induction in adrenal cells: Involvement of the PI3K pathway
(BioScientifica, 2016-08)
In addition to the well-known function of ACTH as the main regulator of adrenal steroidogenesis, we have previously demonstrated its effect on the transcriptional stimulation of HO-1 expression, a component of the cellular ...