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Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography
(ELSEVIER SCIENCE BV, 2007-06)
Hydrophobic interaction chromatography (HIC) is an important technique for protein purification, which exploits the separation of proteins
based on hydrophobic interactions between the stationary phase ligands and hydrophobic ...
Current insights on protein behaviour in hydrophobic interaction chromatography
(2007)
This paper gives a summary of different aspects for predicting protein behaviour in hydrophobic interaction chromatography (HIC). First, a brief description of HIC, hydrophobic interactions, amino acid and protein ...
Predicting the behaviour of proteins in hydrophobic interaction chromatography: 2. Using a statistical description of their surface amino acid distribution
(2006)
This paper focuses on the prediction of the dimensionless retention time (DRT) of proteins in hydrophobic interaction chromatography (HIC) by means of mathematical models based on the statistical description of the amino ...
Predicting the behaviour of proteins in hydrophobic interaction chromatography 1: Using the hydrophobic imbalance (HI) to describe their surface amino acid distribution
(ELSEVIER, 2006-02-24)
This paper focuses on the prediction of the dimensionless retention time of proteins (DRT) in hydrophobic interaction chromatography (HIC) by means of mathematical models based on characteristics of the surface hydrophobicity ...
Application of switchable hydrophobicity solvents for extraction of emerging contaminants in wastewater samples
(MDPI, 2020)
In the present work, the effectiveness of switchable hydrophobicity solvents (SHSs) as extraction solvent (N,N-Dimethylcyclohexylamine (DMCA), N,N-Diethylethanamine (TEA), and N,N-Benzyldimethylamine (DMBA)) for a variety ...
Methane combustion over Pd/SiO2 catalysts with different degrees of hydrophobicity
(ELSEVIER SCIENCE BV, 2005-04-08)
The combustion of methane has been studied over 1% Pd catalysts supported on commercial silica with different hydrophobicity, prepared by chlorine free precursors. It was found that, when no water is added to the feed, the ...
A theory of protein-resin interaction in hydrophobic interaction chromatography
(ELSEVIER SCIENCE, 2005-02-25)
Docking simulations were performed in order to investigate surface area of interaction between several ribonucleases and a reduced model for the hydrophobic moiety used in Phenyl Sepharose(TM) using the program AutoDock ...
Poly(ethylene oxide)s hydrophobically modified. Adsorption and spreading at the air-water interface
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2006-07-01)
A comparative study of spread and adsorbed monolayer of poly(ethylene oxide)s of different molecular weight hydrophobically modified with alkyl isocyanates of different length chain is reported. The modification of the ...
Is it possible to predict the average surface hydrophobicity of a protein using only its amino acid composition?
(ELSEVIER SCIENCE BV, 2005-05-20)
Hydrophobicity is one of the most important physicochemical properties of proteins. Moreover, it plays a fundamental role in hydrophobic interaction chromatography, a separation technique that, at present time, is used in ...
Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography
(SPRINGER, 2008-08)
During cationic bed adsorption (EBA), with cutinase with varying length tryptophan tags (WP)(2) and (WP)(4), 33% and 10% of adsorption capacity and 80% and 32% eluted specific activity were observed in relation to wild ...