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Synthetic non-classical luminescence generation by enhanced silica nanophotonics based on nano-bio-FRET
(Royal Society of Chemistry, 2020-05)
Fluorescent silica nanoparticles (NPs-(SiO2-Fluo)) were synthesized based on the classical Storber method for cyanobacteria labelling. Modified mono-coloured SiO2 NPs with fluorescein (Fl) and rhodamine B (RhB)were obtained ...
A change of view: Homologous recombination at single-molecule resolution
(Nature Publishing Group, 2018-04)
Genetic recombination occurs in all organisms and is vital for genome stability. Indeed, in humans, aberrant recombination can lead to diseases such as cancer. Our understanding of homologous recombination is built upon ...
Quantifying transcription factor binding dynamics at the single-molecule level in live cells
(Academic Press Inc Elsevier Science, 2017-07)
Progressive, technological achievements in the quantitative fluorescence microscopy field are allowing researches from many different areas to start unraveling the dynamic intricacies of biological processes inside living ...
Photoswitchable fluorescent diheteroarylethenes: substituent effects on photochromic and solvatochromic properties
(Royal Society of Chemistry, 2014-01)
Photoswitchable fluorescent diheteroarylethenes are promising candidates for applications in super-resolution molecular localization fluorescence microscopy thanks to their high quantum yields and fatigue-resistant ...
New fluorinated rhodamines for optical microscopy and nanoscopy
(Wiley VCH Verlag, 2010-04)
New photostable rhodamine dyes represented by the compounds 1a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, ...
Therapeutic strategies to protect the central nervous system against shiga toxin from enterohemorrhagic escherichia coli
(Bentham Science Publishers, 2020-02)
Infection with Shiga toxin-producing Escherichia coli (STEC) may cause hemorrhagic colitis, hemolytic uremic syndrome (HUS) and encephalopathy. The mortality rate derived from HUS adds up to 5% of the cases, and up to 40% ...
Lateral sensitivity in electron probe microanalysis studied by Monte Carlo simulations involving fluorescence enhancements
(Wiley Blackwell Publishing, Inc, 2018-05-27)
In electron probe microanalysis, secondary fluorescence can occur leading to an increase of the volume analysed, degrading the lateral resolution of this technique. An adequate knowledge of the interaction volumes from ...
Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms
(Cell Press, 2019-04)
Eukaryotic chromosomes are organized in multiple scales, from nucleosomes to chromosome territories. Recently, genome-wide methods identified an intermediate level of chromosome organization, topologically associating ...
Co-imaging extrinsic, intrinsic and effector caspase activity by fluorescence anisotropy microscopy
(Elsevier, 2018-10)
In order to overcome intercellular variability and thereby effectively assess signal propagation in biological networks it is imperative to simultaneously quantify multiple biological observables in single living cells. ...
X-Y sample scanning stage and calibration method suitable for single-molecule detection
(Elsevier Science Sa, 2010-10)
This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out ...