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A Practical Teaching Course in Directed Protein Evolution Using the Green Fluorescent Protein as a Model
(WILEY-BLACKWELL, 2011)
Protein engineering is a powerful tool, which correlates protein structure with specific functions, both in applied biotechnology and in basic research. Here, we present a practical teaching course for engineering the green ...
Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera
(2021-01-01)
In this study, we analyzed Apis mellifera royal jelly proteome alterations induced by supplementation with different concentrations of dietary organic zinc (0, 25, 50, and 75 ppm). Proteins extracted from royal jelly were ...
The promoter of a gene encoding an isoflavone reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves
(Springer, 2009-11-01)
A cDNA clone (designated CaIRL) encoding an isoflavone reductase-like protein from coffee (Coffea arabica) was retrieved during a search for genes showing organ/tissue-specific expression among the expressed sequence tags ...
Fetal-Maternal Interactions in the Synepitheliochorial Placenta Using the eGFP Cloned Cattle Model
(2013-05-28)
Background: To investigate mechanisms of fetal-maternal cell interactions in the bovine placenta, we developed a model of transgenic enhanced Green Fluorescent Protein (t-eGFP) expressing bovine embryos produced by nuclear ...
Prognostic value of NDRG1 and SPARC protein expression in breast cancer patients
(SPRINGER, 2011)
An increasing number of studies have shown altered expression of secreted protein acidic and rich in cysteine (SPARC) and N-myc down-regulated gene (NDRG1) in several malignancies, including breast carcinoma; however, the ...
Maternal protein restriction induce skeletal muscle changes without altering the MRFs MyoD and myogenin expression in offspring
(Springer, 2012-10-01)
Stimuli during pregnancy, such as protein restriction, can affect morphophysiological parameters in the offspring with consequences in adulthood. The phenomenon known as fetal programming can cause short- and long-term ...
Whey protein hydrolysate enhances the exercise-induced heat shock protein (HSP70) response in rats
(Elsevier Sci LtdOxfordInglaterra, 2013)
Refolded dengue virus type 2 NS1 protein expressed in Escherichia coli preserves structural and immunological properties of the native protein
(ELSEVIER SCIENCE BV, 2010)
The dengue virus NS1 protein has been shown to be a protective antigen under different experimental conditions but the recombinant protein produced in bacterial expression systems is usually not soluble and loses structural ...