Artículo
Cooperative platform (facsnanort-qpcr): contribution to the successful management of patients with mrd leukemia
Registro en:
Garcia. A., Rosales, V., Perea, C., Maldonado, M. & Lopez, E.(2013), "COOPERATIVE PLATFORM (FACSNANORT-QPCR): CONTRIBUTION TO THE SUCCESSFUL MANAGEMENT OF PATIENTS WITH MRD LEUKEMIA ", Revista Ideas en Ciencia, 40, pp 42-48
2007-5197
Autor
ALONSO GARCIA, ANA LUCIA; 201218
Rosales Garcia, Victor Hugo;#0000-0002-5204-7789
Perea Montoya, Cinthia;x1335470
Maldonado Calderón, María Teresa;x1335471
López Orduña, Eduardo;x1335472
ALONSO GARCIA, ANA LUCIA
Rosales Garcia, Victor Hugo
Perea Montoya, Cinthia
Maldonado Calderón, María Teresa
López Orduña, Eduardo
Institución
Resumen
t(9;22) Ph translocation is a chromosome abnormality recurrent in human chronic myelogenous leukemia (CML) and cases of childhood acute lymphoblastic leukemia (ALL). Ph is a marker of adverse prognosis significant with remission more frequent and earlier and lower percentage of overall survival. If there are less than 5% blasts in a bone marrow sample, the patient is in "clinical remission". In the light of sensitivity current controls molecular (10-6) are an important role in therapy, although this depends on the amount of cells analyzed. Our goal is to improve the sensitivity by proposing a platforms combination, which allow MRD evaluation with only 10 cells. Flow cytometry sorting (multiparameter immunological detection), nano-PCR (RNA isolation and RT) and qPCR (diagnostic confirmation). The molecular remission has many more probability to be lasting that the cytogenetic remission, both before and after the transplant. We consider this technique as an important step forward towards performed diagnosis, monitoring treatment responses, and identification of relapse in Leukemia patients.