Otro
Model of the tomogram
Autor
Marsh, Brad J.
Howell, Kathryn E.
McIntosh, J. Richard
Resumen
All of the organelles and structures within the tomogram shown in the Dual Axis 3-D Reconstructions movie (6c) were individually traced and modeled with tools in the IMOD software package. Each structure was considered as a separate object and was modeled in a different color. The finished model demonstrates the 3-D complexity of the Golgi and its position in the cytoplasm relative to other organelles and elements of the cytoskeleton. The seven cisternae of the Golgi Complex (named C1-C7 in the cis-to-trans direction) are colored light blue (C1), pink (C2), rose (C3), green (C4), dark blue (C5), amber (C6) and red (C7). When the model is broken down to display subsets of the Golgi cisternae, one can see that they vary considerably in size and that many are reticular. The extent to which adjacent cisternae adhere to one another varies as well. For example, C6 has pulled away slightly from C5 and is extremely reticular. Also, a large tubule extends from C6 to a region distal to the Golgi stack. None of the vesicles or tubules on C6 are clathrin coated. The trans-most cisterna (C7) differs from the previous six cisternae in that it is more 'solid' (less reticular) and all vesicles and tubules budding from it are coated with clathrin. This point is shown more clearly in tomogram 6e, where the C6 and C7 cisternae are isolated Componente Curricular::Educação Superior::Ciências Biológicas::Morfologia