Videos
Retromer-containing tubules leaving early endosomes [4e. Honorable Mention]
Autor
Mardones, Gonzalo A.
Resumen
Presentes the retromer is a multisubunit complex that mediates retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-MPR) from endosomes to the trans-Golgi network (TGN). The CI-MPR binds newly-synthesized acid hydrolases at the TGN and carries them within clathrin-coated vesicles to endosomes, where the hydrolases are released for eventual transport to lysosomes. The retromer then functions to retrieve the unoccupied receptors to the TGN, where they engage in further cycles of acid hydrolase sorting. The mammalian retromer comprises two biogenetically distinct subcomplexes of tightly assembled subunits: a homodimer or heterodimer of SNX1 or SNX2 and a heterotrimer composed of Vps26, Vps29 and Vps35. This video shows a HeLa cell transiently expressing Vps29-GFP. By means of high-speed live-cell imaging the video shows the association of Vps29-GFP to both early endosomes and highly mobile vesicles. It also shows the retromer in tubular elements detaching from early endosomes Componente Curricular::Educação Superior::Ciências Biológicas::Morfologia