Videos
Classic mitosis in a vertebrate cell
Autor
Potapova, Tamara
Gorbsky, Gary
Resumen
Presents a video showing classic mitosis in a Xenopus laevis cell S3 stably expressing GFP alpha-tubulin to visualize dynamics of the microtubule network. It illustrates a complete sequence of mitotic events starting from late G2 with disassembly of nucleoli. Subsequently in prophase, the spindle poles separate, the chromosomes condense and the nuclear envelope breaks down. Later during prometaphase and metaphase, the cell contracts, the mitotic spindle forms, and chromosomes align at the metaphase plate. At the onset of anaphase, the chromatids segregate to opposite poles of the spindle and the cleavage furrow separates the cell in two daughter cells connected by the midbody. In the subsequent telophase cells undergo exit from mitosis, illustrated by chromosome decondensation, formation of nuclear envelope and nucleoli, establishment of the interphase array of microtubules and expansion of the cell cytoplasm on the substrate. In the end, the two daughter cells are in G1 and remain connected by the midbody Componente Curricular::Educação Superior::Ciências Biológicas::Biologia Geral