Videos
PIP3 produced in response to stimulation by a growth factor
Autor
Field, Seth J.
Resumen
Movie of an NIH 3T3 mouse fibroblast that has been starved for growth factors and then stimulated by platelet-derived growth factor (PDGF). The cell is expressing a reporter for PIP3 (phosphatidylinositol(3,4,5)trisphosphate), composed of a PIP3 binding domain (the pleckstrin homology domain of Bruton's Tyrosine Kinase) fused to enhanced green fluorescent protein (EGFP). In the starved cell the reporter is partially nuclear (likely due to a weak nuclear localization sequence within EGFP). Beginning at the third frame, in response to 50ng/ml PDGF, the reporter rapidly reveals the production of the lipid second messenger PIP3 at the plasma membrane Componente Curricular::Educação Superior::Ciências Biológicas::Morfologia