Common problems when carrying out water footprint (WF) assessments are obtaining specific primary data, dealing with the complexity of its computation, and the availability of quality data. In a supply chain context, inconsistencies are even more exacerbated. In order to fill in this research gap, this study proposes and evaluates the content validity of a survey scale to assess WF management initiatives implemented by companies, with a focus on supply chains and the agriculture industry. In order to do so, a literature review was performed to identify candidate survey items whose content was later validated with experts in terms of their relevance, clarity, and essentiality to measure WF management. Content validity was assessed using several indices (items’ content validity index (I-CVI), Kappa’s coefficient, Aiken’ V coefficient, and content validity ratio (CVR)), which indicated high content validity for the selected items. This study provides a set of measurement survey items that can be used to evaluate WF management initiatives implemented in agri-food supply chains in future empirical studies. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.ANID FONDECYT, (3210113); Universidad Autónoma de Chile