Effects of Protein A in Detection of Canine Distemper Virus through immunosensor construction

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBasso, Caroline Rodrigues
dc.creatorSempionatto, Juliane R.
dc.creatorTozato, Claudia C.
dc.creatorCastro, Gustavo Rocha de
dc.creatorAraujo Júnior, João Pessoa
dc.creatorPedrosa, Valber A.
dc.date2016-03-02T13:04:26Z
dc.date2016-10-25T21:33:29Z
dc.date2016-03-02T13:04:26Z
dc.date2016-10-25T21:33:29Z
dc.date2015
dc.date.accessioned2017-04-06T10:07:15Z
dc.date.available2017-04-06T10:07:15Z
dc.identifierIEEE Sensors Journal, v. 15, n. 8, p. 4677-4683, 2015.
dc.identifier1530-437X
dc.identifierhttp://hdl.handle.net/11449/135788
dc.identifierhttp://acervodigital.unesp.br/handle/11449/135788
dc.identifier10.1109/JSEN.2015.2426421
dc.identifier0278054122091940
dc.identifierhttp://dx.doi.org/10.1109/JSEN.2015.2426421
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/946294
dc.descriptionWe have developed a detection system to canine distemper virus (CDV) by employing surface plasmon resonance (SPR) and compared the effect of oriented and random immobilization. Several parameters, such as the surface concentration of immobilized antibodies, antigen binding capacity, and the maximum surface concentration of immobilized antibody were optimized. The maximal antigen binding capacity was obtained by means of a SPR-sensor immobilized via protein A, which leads to an end-on orientation. CDV at 0.1 ng/mL can be detected using protein A. Urine analysis showed that all samples contained detectable levels of CDV, but the results revealed an improvement in sensitivity of the assay when it is specifically oriented. The described method is simple, fast, selective, and sensitive to the biorecognition element. The results emphasize how important it is to attach proteins on surfaces in such a way that their binding sites are oriented toward the solution phase.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.relationIEEE Sensors Journal
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntibody
dc.subjectCanine distemper virus
dc.subjectImmunosensor
dc.subjectOriented immobilization
dc.subjectProtein A
dc.titleEffects of Protein A in Detection of Canine Distemper Virus through immunosensor construction
dc.typeOtro


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