Otro
Detection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumour
Registro en:
Veterinary and Comparative Oncology, v. 9, n. 4, p. 251-259, 2011.
1476-5810
10.1111/j.1476-5829.2010.00260.x
4584674909952477
2850453183724361
6404176495306171
4272904699684196
Autor
Stockman, Daniela
Ferrari, Heitor Flávio
Andrade, Alexandre Lima de
Cardoso, Tereza Cristina
Luvizotto, Maria Cecília Rui
Resumen
Canine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT-PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl-2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis.