CD95 (FAS) and CD178 (FASL) induce the apoptosis of CD4+ and CD8+ cells isolated from the peripheral blood and spleen of dogs naturally infected with Leishmania spp

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSilva, Kathlenn Liezbeth Oliveira
dc.creatorMelo, Larissa Martins
dc.creatorPerosso, Juliana
dc.creatorOliveira, Bruna Brito
dc.creatorSantos, Paulo Sérgio Patto dos
dc.creatorEugênio, Flávia de Rezende
dc.creatorLima, Valéria Marçal Felix de
dc.date2016-01-28T16:53:40Z
dc.date2016-10-25T21:27:17Z
dc.date2016-01-28T16:53:40Z
dc.date2016-10-25T21:27:17Z
dc.date2013
dc.date.accessioned2017-04-06T09:44:56Z
dc.date.available2017-04-06T09:44:56Z
dc.identifierVeterinary Parasitology, v. 197, n. 3-4, p. 470-476, 2013.
dc.identifier0304-4017
dc.identifierhttp://hdl.handle.net/11449/133110
dc.identifierhttp://acervodigital.unesp.br/handle/11449/133110
dc.identifier10.1016/j.vetpar.2013.07.012
dc.identifier1128699666207940
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2013.07.012
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/943641
dc.descriptionInfected dogs are urban reservoirs of Leishmania chagasi, which is a causative agent of visceral leishmaniasis (VL). Dogs exhibit immune suppression during the course of this disease, and lymphocyte apoptosis is involved in this process. To investigate apoptosis and the expression levels of FAS-FAS-associated death domain protein (CD95 or APO-1), FASL-FAS ligand protein (CD178), and TRAIL-TNF-related apoptosis-inducing ligand (CD253) receptors in peripheral blood mononuclear cells and spleen leukocytes from 38 symptomatic dogs with moderate VL and 25 healthy dogs were evaluated by flow cytometry. The apoptosis rate of blood and splenic CD4+ and CD8+ cells was higher in infected dogs than in healthy dogs. The expression levels of FAS and FASL in blood and splenic CD4+ cells were lower in infected dogs than in healthy dogs. FAS expression in CD8+ cells was higher in infected dogs than in healthy dogs; in contrast, FASL expression was lower in infected dogs. The expression of the TRAIL receptor increased only in splenic CD8+ cells from infected dogs. The FAS and FAS-L blocking antibodies confirmed the importance of these receptors in apoptosis. Our results enhance the current understanding of the immune response in dogs infected with L. chagasi, facilitating the future development of therapeutic interventions to reduce lymphocyte depletion.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationVeterinary Parasitology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCell death
dc.subjectFAS-associated death domain protein
dc.subjectFASL-FAS ligand protein
dc.subjectLeishmania chagasi
dc.subjectLeishmaniasis
dc.subjectTRAIL (TNF-related apoptosis-inducing ligand)
dc.titleCD95 (FAS) and CD178 (FASL) induce the apoptosis of CD4+ and CD8+ cells isolated from the peripheral blood and spleen of dogs naturally infected with Leishmania spp
dc.typeOtro


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