Otro
A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis
Registro en:
Proceedings Of The National Academy Of Sciences Of The United States Of America, p. 14054–14059, 2015.
1091-6490
10.1073/pnas.1515024112
0000-0002-3034-6497
26494285
Autor
Pedrolli, Danielle Biscaro
Kühm, Christian
Sévin, Daniel C.
Vockenhuber, Michael P.
Sauer, Uwe
Suess, Beatrix
Mack, Matthias
Resumen
Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for biosynthesis and/or transport of riboflavin (rib genes). Cytoplasmic riboflavin is rapidly and almost completely converted to FMN by flavokinases. When cytoplasmic levels of FMN are sufficient (high levels), FMN binding to FMN riboswitches leads to a reduction of rib gene expression. We report here that the protein RibR counteracts the FMN-induced turn-off activities of both FMN riboswitches in Bacillus subtilis, allowing rib gene expression even in the presence of high levels of FMN. The reason for this secondary metabolic control by RibR is to couple sulfur metabolism with riboflavin metabolism.