A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorForesti, F.
dc.creatorOliveira, C.
dc.creatorForesti de Almeida-Toledo, L.
dc.date2014-05-27T11:17:30Z
dc.date2016-10-25T21:21:14Z
dc.date2014-05-27T11:17:30Z
dc.date2016-10-25T21:21:14Z
dc.date1993-09-15
dc.date.accessioned2017-04-06T09:22:13Z
dc.date.available2017-04-06T09:22:13Z
dc.identifierExperientia, v. 49, n. 9, p. 810-813, 1993.
dc.identifier0014-4754
dc.identifierhttp://hdl.handle.net/11449/130469
dc.identifierhttp://acervodigital.unesp.br/handle/11449/130469
dc.identifier10.1007/BF01923555
dc.identifierWOS:A1993LY25500015
dc.identifier2-s2.0-0027366658
dc.identifierhttp://dx.doi.org/10.1007/BF01923555
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/941012
dc.descriptionThis paper describes a new technique for preparing mitotic fish chromosomes using short-term in vitro treatment with colchicine. The results show that a large number of good quality metaphases (many suitable for chromosome banding) can be obtained by this technique, which requires an average of 1 h and 30 min for all steps. The procedure considerably reduces the time normally required for chromosome preparations in fish.
dc.languageeng
dc.publisherBirkhauser Verlag Ag
dc.relationExperientia
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectfish cytogenetics
dc.subjectmitotic chromosomes
dc.subjecttechnique
dc.subjectcolchicine
dc.subjectanimal tissue
dc.subjectchromosome
dc.subjectcytogenetics
dc.subjectfish
dc.subjectmitosis
dc.subjectnonhuman
dc.subjectpriority journal
dc.titleA method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine
dc.typeOtro


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