Otro
A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Registro en:
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.
1517-8382
S1517-83822015000100113
WOS:000355769900013
S1517-83822015000100113.pdf
Autor
Angelini, Leticia Mara Lima
Silva, Amanda Ribeiro Martins da
Rocco, Lucas de Freitas Coli
Milagre, Cintia Duarte de Freitas
Resumen
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)