Maternal protein restriction increases respiratory and sympathetic activities and sensitizes peripheral chemoreflex in male rat offspring

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAlves, José Luiz de Brito
dc.creatorNogueira, Viviane Oliveira
dc.creatorCavalcanti Neto, Marinaldo Pacífico
dc.creatorLeopoldino, Andréia Machado
dc.creatorCurti, Carlos
dc.creatorColombari, Débora Simões de Almeida
dc.creatorColombari, Eduardo
dc.creatorWanderley, Almir Gonçalves
dc.creatorLeandro, Carol Gois
dc.creatorZoccal, Daniel Breseghello
dc.creatorCosta-Silva, João Henrique da
dc.date2015-08-06T16:13:00Z
dc.date2016-10-25T20:53:35Z
dc.date2015-08-06T16:13:00Z
dc.date2016-10-25T20:53:35Z
dc.date2015
dc.date.accessioned2017-04-06T08:36:01Z
dc.date.available2017-04-06T08:36:01Z
dc.identifierThe Journal of Nutrition, v. 145, n. 5, p. 907-914, 2015.
dc.identifier0022-3166
dc.identifierhttp://hdl.handle.net/11449/125744
dc.identifierhttp://acervodigital.unesp.br/handle/11449/125744
dc.identifierhttp://dx.doi.org/10.3945/jn.114.202804
dc.identifier1684467594359403
dc.identifierhttp://jn.nutrition.org/content/145/5/907
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/936337
dc.descriptionBackground: Maternal protein restriction in rats increases the risk of adult offspring arterial hypertension through unknown mechanisms. Objectives: The aims of the study were to evaluate the effects of a low-protein (LP) diet during pregnancy and lactation on baseline sympathetic and respiratory activities and peripheral chemoreflex sensitivity in the rat offspring. Methods: Wistar rat dams were fed a control [normal-protein (NP); 17% protein] or an LP (8% protein) diet during pregnancy and lactation, and their male offspring were studied at 30 d of age. Direct measurements of baseline arterial blood pressure (ABP), heart rate (HR), and respiratory frequency (Rf) as well as peripheral chemoreflex activation (potassium cyanide: 0.04%) were recorded in pups while they were awake. In addition, recordings of the phrenic nerve (PN) and thoracic sympathetic nerve (tSN) activities were obtained from the in situ preparations. Hypoxia-inducible factor 1α (HIF-1α) expression was also evaluated in carotid bifurcation through a Western blotting assay. Results: At 30 d of age, unanesthetized LP rats exhibited enhanced resting Rf (P = 0.001) and similar ABP and HR compared with the NP rats. Despite their similar baseline ABP values, LP rats exhibited augmented low-frequency variability (∼91%; P = 0.01). In addition, the unanesthetized LP rats showed enhanced pressor (P = 0.01) and tachypnoeic (P = 0.03) responses to peripheral chemoreflex activation. The LP rats displayed elevated baseline tSN activity (∼86%; P = 0.02) and PN burst frequency (45%; P = 0.01) and amplitude (53%; P = 0.001) as well as augmented sympathetic (P = 0.01) and phrenic (P = 0.04) excitatory responses to peripheral chemoreflex activation compared with the NP group. Furthermore, LP rats showed an increase of ∼100% in HIF-1α protein density in carotid bifurcation compared with NP rats. Conclusion: Sympathetic-respiratory overactivity and amplified peripheral chemoreceptor responses, potentially through HIF-1α–dependent mechanisms, precede the onset of hypertension in juvenile rats exposed to protein undernutrition during gestation and lactation.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationThe Journal of Nutrition
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleMaternal protein restriction increases respiratory and sympathetic activities and sensitizes peripheral chemoreflex in male rat offspring
dc.typeOtro


Este ítem pertenece a la siguiente institución