Immunohistochemical and molecular analysis of caveolin-1 expression in canine mammary tumors

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorZuccari, Debora Aparecida Pires de Campos
dc.creatorCastro, R.
dc.creatorGavioli, A. F.
dc.creatorMancini, U. M.
dc.creatorFrade, C. S.
dc.creatorLeonel, Camila
dc.date2015-03-18T15:56:25Z
dc.date2016-10-25T20:35:38Z
dc.date2015-03-18T15:56:25Z
dc.date2016-10-25T20:35:38Z
dc.date2012-01-01
dc.date.accessioned2017-04-06T07:18:54Z
dc.date.available2017-04-06T07:18:54Z
dc.identifierGenetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 1, p. 153-165, 2012.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/117556
dc.identifierhttp://acervodigital.unesp.br/handle/11449/117556
dc.identifier10.4238/2012.January.27.2
dc.identifierWOS:000301923500017
dc.identifierWOS000301923500017.pdf
dc.identifierhttp://dx.doi.org/10.4238/2012.January.27.2
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/928203
dc.descriptionCaveolin-1 (Cav-1) is a structural protein present in invaginations of the cell membrane. In human breast cancer, the cav-1 gene is believed to be a tumor suppressor gene associated with inhibition of tumor metastasis. However, little is known about its expression, regulation and function in canine mammary tumors. Expression levels of cav-1 were investigated using real- time PCR and immunohistochemical detection with an anti-human Cav- 1 antibody. Gene expression stability of different samples was analyzed using the geNorm software. Mammary tumors from 51 female dogs were compared to normal mammary tissue from 10 female dogs. Malignant mammary cells showed a loss of Cav-1 expression by quantitative RTPCR and weak Cav-1 staining by immunohistochemistry compared to normal mammary gland tissue. There was a significant relationship between outcome and immunostaining as well as with tumor size, indicating that caveolin subexpression has a positive predictive value and is related to higher survival and smaller tumor size. Our findings indicate that Cav-1 is a potential prognostic marker for canine mammary tumors.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherFunpec-editora
dc.relationGenetics And Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMammary cancer
dc.subjectPrognosis
dc.subjectImmunohistochemistry
dc.subjectQuantitative PCR
dc.titleImmunohistochemical and molecular analysis of caveolin-1 expression in canine mammary tumors
dc.typeOtro


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