Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

Aguirre, Carlos; Olivares, Natalia; Hinrichsen, Patricio; Sanchez, Evelyn [Univ Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Chile]

Artículo o Paper

Fecha

2021-06-04

Registro en:

Aguirre, C., Sánchez, E., Olivares, N., & Hinrichsen, P. (2021). Multiplex TaqMan real-time PCR assay for sensitive detection of two weevil species (Coleoptera: Curculionidae). Journal of Economic Entomology, 114(1), 90-99.

0022-0493

eISSN: 2309-5807

WOS: 000648959000010

PMID: 33155656

https://repositorio.umayor.cl/xmlui/handle/sibum/9048

https://doi.org/10.1093/jee/toaa251

https://academic.oup.com/jee/article-pdf/114/1/90/36217077/toaa251.pdf

https://watermark.silverchair.com/toaa251.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA18wggNbBgkqhkiG9w0BBwagggNMMIIDSAIBADCCA0EGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMmg5Xol_l7494cNhsAgEQgIIDEu--tiA04Mn7iaiVTwqKfMTWH1f4KumN_kkhqO6A2_y1nbdOLlstu-zFlx83Mn-cJaa8dmAaX3jQiB9-OhCHrb3jp4HTIQVz4T2-Ks8nP5rrocEB7CJdIY1MYGu8DtfAPRltmQ8yU3JenzrblHvbGsKhUgKVq6gzlKyEwrDMgz8eV1pROeuLbGGvkJWnJ1vLIQIRap4keOl1DTBkf7rv1GDZUP7QKmPWml8aMlDwSfKHksdaQXHBdnu8pJu_sh8c-vA2e7i_somU2lEQfUu0o2dzogHG5vx_s-lYcZOQ_-iPQYRYRTmOa6BhiFyXzdY_-ONQkHfIyXFFRTbFU9eOExDa_lHUYbAslWQPd4FPz4hKmYHfhTNYW4njjJRwPi6FTp7RvXy7iajWZ3Bx1BjxAmh8-Tr5kHYl6wLchT1Svj0pZMEtA0YCEbqCBPk-GNxgwJPy0iE5kv5VkpwU5Es7K5CJmQ7SxuVe6yGd2_RscOXz9RMZHoiv7UDUMLQVMtHglusiFI_TJ0P9kVdVHdJS0aaNo9yI_faT07d74iBN05fcKYprhj3MRy5gVcQdZUZPnTkqzIPA1rz9RIhW147uHrmhA3-S1feroBRN1ZpFaft1owVpvQWWvC1t3wofqaG4qKRIhaSELjnyNfaI3b1msVZtprX_E1F5Y2BeRxgOB5yhnHsx9rPB4diG8QW7TzxZU9zkmgrjryPyggusluwFz-R58Pr7yq14KCIEty-mJL8pfJezX7YJLbPVuTxOU9n6Fk3xTQ7SwNNSVReux9heILnjCjgKywouBdzMtmzbB9bQyMDgn8-DjR3qFYdJRSOLaHQvQZOctm_OB-GupcL3uVhbAhSeFWbfe-dUdSjUF2IaB9J8QwjuyVDiLNQth269-uWqG7xg4Iv2kJWdGIeAyusvs1Qt1cm0DcrH_cff-D-3IZqJ4R4s7_kzB81JpS4M3-DGhI8z3hcf3TqmTxt2v5sJD2rxfUUoglGJcCccgNgLSTLNiUfd0rxOyS0vBplQnTEu3q0kY2_NKRQGjaAG1THp6g

10.1093/jee/toaa251

https://repositorioslatinoamericanos.uchile.cl/handle/2250/9275834

Autor

Aguirre, Carlos

Olivares, Natalia

Hinrichsen, Patricio

Sanchez, Evelyn [Univ Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Chile]

Institución

Universidad Mayor (Chile)

Resumen

Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus. This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/mu l to 1 x 10(-6) pg/mu l, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.

Materias

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