Otro
Balanced anesthesia with sevoflurane does not alter redox status in patients undergoing surgical procedures
Registro en:
Mutation Research-genetic Toxicology And Environmental Mutagenesis. Amsterdam: Elsevier Science Bv, v. 773, p. 29-33, 2014.
1383-5718
10.1016/j.mrgentox.2014.07.007
WOS:000343948900005
0000-0003-4413-226X
Autor
Orosz, Jose Eduardo B.
Braz, Leandro Gobbo
Ferreira, Ana Lúcia dos Anjos
Amorim, Rosa Beatriz
Salvadori, Daisy Maria Favero
Yeum, Kyung-Jin
Braz, José Reinaldo Cerqueira
Braz, Mariana Gobbo
Resumen
Despite the effectiveness and safety of anesthetics, some unanswered questions remain concerning their toxicity and effects on cellular redox balance. To test for possible toxic effects of balanced anesthesia maintained with the volatile anesthetic sevoflurane, we evaluated oxidative stress during and after general anesthesia in 15 adult patients without comorbidities who underwent elective minor surgical procedures. Venous blood samples were collected at baseline, before anesthesia (t0); after anesthesia induction and immediately before surgery (t1); 2 h after the beginning of anesthesia (t2); and on the day following surgery (t3). Antioxidant defense was determined by fluorometry. Oxidative stress markers included oxidative DNA damage, evaluated by the alkaline comet assay, and plasma malondialdehyde (MDA), assessed by high performance liquid chromatography (HPLC). No increase in oxidized DNA damage or antioxidant defense was observed. Plasma MDA increased only at t3 compared with t2. Balanced sevoflurane-maintained anesthesia appears neither to damage DNA nor to alter redox status. (C) 2014 Elsevier B.V. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)