artículo
The 3 ' Untranslated Region of the Andes Hantavirus Small mRNA Functionally Replaces the Poly(A) Tail and Stimulates Cap-Dependent Translation Initiation from the Viral mRNA
Fecha
2010Registro en:
10.1128/JVI.01270-10
1098-5514
0022-538X
MEDLINE:20660206
WOS:000282641800073
Autor
Vera Otarola, Jorge
Soto Rifo, Ricardo
Ricci, Emiliano P.
Ohlmann, Theophile
Darlix, Jean Luc
Lopez Lastra, Marcelo
Institución
Resumen
In the process of translation of eukaryotic mRNAs, the 5' cap and the 3' poly(A) tail interact synergistically to stimulate protein synthesis. Unlike its cellular counterparts, the small mRNA (SmRNA) of Andes hantavirus (ANDV), a member of the Bunyaviridae, lacks a 3' poly(A) tail. Here we report that the 3' untranslated region (3'UTR) of the ANDV SmRNA functionally replaces a poly(A) tail and synergistically stimulates cap-dependent translation initiation from the viral mRNA. Stimulation of translation by the 3'UTR of the ANDV SmRNA was found to be independent of viral proteins and of host poly(A)-binding protein.