Enhancing effect of lysine combined with other compounds on cephamycin C production in Streptomyces clavuligerus

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLeite, Carla A.
dc.creatorCavallieri, Andre P.
dc.creatorAraujo, Maria Lucia Gonsales da Costa
dc.date2014-12-03T13:11:27Z
dc.date2016-10-25T20:14:14Z
dc.date2014-12-03T13:11:27Z
dc.date2016-10-25T20:14:14Z
dc.date2013-12-20
dc.date.accessioned2017-04-06T06:30:25Z
dc.date.available2017-04-06T06:30:25Z
dc.identifierBmc Microbiology. London: Biomed Central Ltd, v. 13, 11 p., 2013.
dc.identifier1471-2180
dc.identifierhttp://hdl.handle.net/11449/113151
dc.identifierhttp://acervodigital.unesp.br/handle/11449/113151
dc.identifier10.1186/1471-2180-13-296
dc.identifierWOS:000330076600002
dc.identifierWOS000330076600002.pdf
dc.identifierhttp://dx.doi.org/10.1186/1471-2180-13-296
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/923902
dc.descriptionBackground: Lysine plays an important role in Streptomyces clavuligerus metabolism; it takes part in its catabolism, via cadaverine, and in its secondary metabolism, in which lysine is converted via 1-piperideine-6-carboxylate to alpha-aminoadipic acid, a beta-lactam antibiotic precursor. The role of lysine as an enhancer of cephamycin C production, when added to production medium at concentrations above 50 mmol l(-1), has already been reported in the literature, with some studies attributing a positive influence to multifunctional diamines, among other compounds. However, there is a lack of research on the combined effect of these compounds on antibiotic production.Results: Results from experimental design-based tests were used to conduct response surface-based optimization studies in order to investigate the synergistic effect of combining lysine with cadaverine, putrescine, 1,3-diaminopropane, or alpha-aminoadipic acid on cephamycin C volumetric production. Lysine combined with cadaverine influenced production positively, but only at low lysine concentrations. On the whole, higher putrescine concentrations (0.4 g l(-1)) affected negatively cephamycin C volumetric production. In comparison to culture media containing only lysine as additive, combinations of this amino acid with alpha-aminoadipic acid or 1,3-diaminopropane increased cephamycin C production by more than 100%.Conclusion: This study demonstrated that different combinations of lysine with diamines or lysine with alpha-aminoadipic acid engender significant differences with respect to antibiotic volumetric production, with emphasis on the benefits observed for lysine combined with alpha-aminoadipic acid or 1,3-diaminopropane. This increase is explained by mathematical models and demonstrated by means of bioreactor cultivations. Moreover, it is consistent with the positive influence of these compounds on lysine conversion to alpha-aminoadipic acid, a limiting step in cephamycin C production.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherBiomed Central Ltd.
dc.relationBMC Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectStreptomyces clavuligerus
dc.subjectCephamycin C
dc.subjectLysine
dc.subjectDiamines
dc.subjectAlpha-aminoadipic acid
dc.subjectResponse surface
dc.titleEnhancing effect of lysine combined with other compounds on cephamycin C production in Streptomyces clavuligerus
dc.typeOtro


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