Colombia
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Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit
Registro en:
Olmo F, Escobedo-Orteg J, Palma P, Sánchez-Moreno M, Mejía-Jaramillo A, Triana O, Marín C. Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit. Asian Pac J Trop Med. 2014 Nov;7(11):854-9. doi: 10.1016/S1995-7645(14)60149-8.
1995-7645
10.1016/S1995-7645(14)60149-8
2352-4146
Autor
Mejía Jaramillo, Ana María
Olmo, Francisco
Escobedo Ortegón, Javier
Palma, Patricia
Sánchez Moreno, Manuel
Triana Chávez, Omar
Clotilde, Marín
Institución
Resumen
ABSTRACT: Objective: To classify 21 new isolates of Trypanosoma cruzi (T. cruzi) according to the Discrete
Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to
detect the parasite in biological samples. Methods: Strains were isolated, DNA extracted, and
classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used
along with other isolates of various biological samples, for a new PCR using primers designed.
Finally, the amplified fragments were sequenced. Results: It was observed the predominance
of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no
band was obtained when other species were used. Conclusions: This work reveals the genetic
variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for
detecting the presence of T. cruzi. COL0007865