Continuous vs. segmented second-dimension system gradients for comprehensive two-dimensional liquid chromatography of sugarcane (Saccharum spp.)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLeme, Gabriel Mazzi
dc.creatorCacciola, Francesco
dc.creatorDonato, Paola
dc.creatorCavalheiro, Alberto José
dc.creatorDugo, Paola
dc.creatorMondello, Luigi
dc.date2014-12-03T13:08:40Z
dc.date2016-10-25T20:08:36Z
dc.date2014-12-03T13:08:40Z
dc.date2016-10-25T20:08:36Z
dc.date2014-07-01
dc.date.accessioned2017-04-06T06:13:16Z
dc.date.available2017-04-06T06:13:16Z
dc.identifierAnalytical And Bioanalytical Chemistry. Heidelberg: Springer Heidelberg, v. 406, n. 18, p. 4315-4324, 2014.
dc.identifier1618-2642
dc.identifierhttp://hdl.handle.net/11449/111457
dc.identifierhttp://acervodigital.unesp.br/handle/11449/111457
dc.identifier10.1007/s00216-014-7786-8
dc.identifierWOS:000338722400001
dc.identifierhttp://dx.doi.org/10.1007/s00216-014-7786-8
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/922236
dc.descriptionA comprehensive two-dimensional liquid chromatography system in combination with photodiode array and mass spectrometry detection was developed for analysis of polyphenols in sugarcane (Saccharum spp.) leaf extracts. To achieve this, a micro cyano column and a partially porous octodecylsilica column were used in the first and the second dimension, respectively. The choice of the cyano column over other reversed-phase columns tested for the first-dimension separation was due to its lower correlation selectivity with respect to the octodecylsilica column, which was used for the second-dimension separation. Even when reversed-phase mode was used in both dimensions, a satisfactory degree of orthogonality was achieved by use of different gradient elution modes in the second dimension. By means of the setup investigated, 38 polyphenolic compounds were detected, and among them 24 were positively identified by means of complementary data from photodiode array and mass spectrometry detection and an in-house database. This is the first time such a powerful analytical technique has been used for polyphenolic characterization of sugarcane extracts.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSpringer
dc.relationAnalytical and Bioanalytical Chemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPolyphenols
dc.subjectComprehensive two-dimensional liquid chromatography
dc.subjectMass spectrometry
dc.subjectCyano column
dc.subjectSugarcane
dc.titleContinuous vs. segmented second-dimension system gradients for comprehensive two-dimensional liquid chromatography of sugarcane (Saccharum spp.)
dc.typeOtro


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